PXD009823 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chromatin phosphoproteomics unravels a function for AT-hook motif nuclear localized protein AHL13 in PAMP-triggered immunity |
Description | Mitogen-activated protein kinases constitute essential signaling mechanisms linking external signal perception to gene expression in all eukaryotes. In Arabidopsis, MAPKs are found in the cytoplasmic and nuclear compartments, but no nuclear phosphoproteomic studies of mapk mutants were performed so far. In this work, we report a panel of nuclear and chromatin-associated targets by performing phosphoproteome analyses of wild-type and mpk3, mpk4, and mpk6 mutant plants. Since the three MAPKs play a role in cell division, development and innate immunity, we also analyzed the phosphoproteomes following microbe-associated molecular pattern (MAMP) treatment. A total of 165 proteins were identified to be differentially phosphorylated when compared between genotypes and in response to MAMP treatment. Using different criteria, including different biochemical and interaction assays, a number of the putative MAPK substrates were validated as true substrates for different MAPKs. Signaling networks for the three MAPKs revealed their common role in RNA transcription, RNA processing and chromatin organization, but also specific roles in development and organelle organization. Overall, this study unravels a set of chromatin-associated targets for these three MAPKs and promises novel insights into yet undiscovered domains linking MAPK signaling to a number of chromatin-related events. |
HostingRepository | PRIDE |
AnnounceDate | 2021-01-11 |
AnnouncementXML | Submission_2021-01-11_11:15:48.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD009823 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Delphine PFLIEGER |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-05-18 01:17:57 | ID requested | |
1 | 2021-01-07 06:35:42 | announced | |
⏵ 2 | 2021-01-11 11:15:48 | announced | 2021-01-11: Updated project metadata. |
3 | 2021-01-11 11:53:21 | announced | 2021-01-11: Updated project metadata.
2021-01-11: Updated publication reference for DOI(s): 10.1073/pnas.2004670118. |
4 | 2023-11-14 08:49:55 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Arabidopsis thaliana, immune MAPKs, phosphoproteomics |
Contact List
Heribert Hirt |
contact affiliation | KAUST |
contact email | heribert.hirt@kaust.edu.sa |
lab head | |
Delphine PFLIEGER |
contact affiliation | CNRS |
contact email | delphine.pflieger@cea.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD009823 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009823
- Label: PRIDE project
- Name: Chromatin phosphoproteomics unravels a function for AT-hook motif nuclear localized protein AHL13 in PAMP-triggered immunity