PXD009813 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human salivary basic proline-rich proteins |
Description | Human basic proline-rich proteins and basic glycosylated proline-rich proteins are the most complex family of human adult salivary proteins, secreted only by the parotid gland, expressed by the polymorphic PRB1-4 genes. This study reports the results obtained for their characterization in our laboratory utilizing integrated top-down and bottom-up HPLC-ESI-MS and MS/MS platform. The family include 11 parent peptides/proteins and more than 6 parent glycosylated proteins. Despite the complexity, on the basis of their primary structure they result the product of the duplication of three repeats and we propose that they can be classified in two main groups, the first including the peptides called P-E, P-Ko, IB-6, Ps-1, Ps-2, P-H, P-F, P-J, and P-D, the second including the peptides called IB-1, II-2 and the glycosylated basic proline-rich proteins codified by PRB3 and PRB4 genes. A third minor hybrid group includes the proteins called IB-8a Con1- and Con1+. Some of the proteins of the family (IB-1, II-2 and P-J) are almost always detectable in adult human saliva, while the others are expressed with different frequencies according to the phenotype. Various isoforms are detectable in the adult saliva and three of them, until now not reported (P-H S1→A, P-Ko P36→S, P-Ko A41→S), were characterized by MS/MS fragmentation. |
HostingRepository | PRIDE |
AnnounceDate | 2018-08-08 |
AnnouncementXML | Submission_2018-08-08_09:47:41.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Barbara Manconi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-05-17 08:10:10 | ID requested | |
⏵ 1 | 2018-08-08 09:47:43 | announced | |
Publication List
Padiglia A, Orr, ù R, Boroumand M, Olianas A, Manconi B, Sanna MT, Desiderio C, Iavarone F, Liori B, Messana I, Castagnola M, Cabras T, Extensive Characterization of the Human Salivary Basic Proline-Rich Protein Family by Top-Down Mass Spectrometry. J Proteome Res, 17(9):3292-3307(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Human, Saliva, High-Resolution ESI−MS/MS Experiments, |
Contact List
Tiziana Cabras |
contact affiliation | Cagliari University |
contact email | tcabras@unica.it |
lab head | |
Barbara Manconi |
contact affiliation | University of Cagliari |
contact email | bmanconi@unica.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009813
- Label: PRIDE project
- Name: Human salivary basic proline-rich proteins