PXD009812

PXD009812 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis in Arabidopsis tissues reveals hydrogen cyanide regulation of intracellular processes through posttranslational modification of proteins by S-cyanylation
Description	Hydrogen cyanide (HCN) is co-produced with ethylene in plant cell and enzymatically detoxified mainly by the mitochondrial ß-cyanoalanine synthase (CAS-C1). Permanent or transient depletion of CAS-C1 activity in Arabidopsis results in physiological alteration in the plant that point to the function of HCN as a gasotransmitter molecule. Label-free quantitative proteomic analysis of enriched mitochondrial samples isolated from wild type and cas-c1 mutant reveled significant changes in protein content, identifying 451 proteins that are absent or less abundant in cas-c1 and 353 proteins only present or more abundant in the mutant background. Gene onthology classification of these proteins highlights proteomic changes that explains the root hairless phenotype and the altered inmune response observed in cas-c1 mutant. The mechanism of action of cyanide as signaling molecule has been addressed by two proteomic approaches focused on identifying S-cyanylation of cysteine as a posttranslational modification of proteins. Both the 2-imino-thiazolidine chemical method and direct untargeted analysis of proteins by LC-MS/MS identified a set of 163 proteins susceptibles to be S-cyanylated that include sedoheptulose 1, 7 biphosphatase (SBPase), the peptidyl-prolyl cis-trans isomerase (CYP20-3) and enolase 2 (ENO2). In vitro analysis of these proteins identified this modification in SBPase Cys74, CYP20-3 Cys259 and ENO2 Cys346 residues that affected the enzymatic activity of the enzymes. GO classification and protein-protein interaction cluster analysis revealed the function of S-cyanylation in the regulation of primary metabolic pathways as glycolysis, and the Calvin and S-adenosylmethionine cicles.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:10:36.297.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luis C. Romero
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	S-cyano-L-cysteine; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-17 07:59:20	ID requested
1	2019-01-09 02:44:05	announced
⏵ 2	2024-10-22 04:10:37	announced	2024-10-22: Updated project metadata.

Garc, í, a I, Arenas-Alfonseca L, Moreno I, Gotor C, Romero LC, cyanylation. Plant Physiol, 179(1):107-123(2019) [pubmed]

10.1104/pp.18.01083;

curator keyword: Biological

submitter keyword: Arabidopsis root cyanylation cyanoalanine synthase

Luis C Romero
contact affiliation	Instituto de Bioquimica Vegetal y Fotosintesis. CSIC
contact email	lromero@ibvf.csic.es
lab head
Luis C. Romero
contact affiliation	Instituto Bioquimica Vegetal y Fotosintesis-CSIC
contact email	lromero@ibvf.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009812
     1. Label: PRIDE project
     2. Name: Proteomic analysis in Arabidopsis tissues reveals hydrogen cyanide regulation of intracellular processes through posttranslational modification of proteins by S-cyanylation