PXD009810

PXD009810 is an original dataset announced via ProteomeXchange.

Title	Sex-dependent variation of pumpkin (Cucurbita maxima cv Big Max) nectar and nectaries as determined by proteomics and metabolomics
Description	Cucurbita maxima c.v. Big Max is a monoecious plant with separate male and female flowers which utilizes nectar as a floral reward for the attraction of bees to the flowers. Maintenance of this mutualism with bees is essential for the reproductive success in this economically valuable crop. In this study, the sex-dependent variation in composition of male and female nectar and the nectaries were defined using a combination of GC-MS based metabolomics and LC-MS/MS based proteomics. Nectar proteins were detected non-quantitatively using LC-MS/MS coupled to Orbitrap. The male and female nectary proteomes were quantified using iTRAQ labeled peptides which were fractioned and concatenated prior to detection using LC-MS/MS coupled to Orbitrap. The nectar proteome consists of 46 proteins, of which 70% overlap between nectar types. Only two proteins are unique to female nectar, and 10 are specific to male nectar. Nectarins (nectar proteins) commonly alter nectar carbohydrates. In our datasets, significant differences in carbohydrate abundance, specifically galactose and sucrose, may be explained by the unique presence of galactinol--sucrose galactosyltransferase 2 (functioning in galactose metabolism) and invertase (catalyzes the hydrolysis of sucrose to glucose and fructose) in the nectar of female and male flowers respectively. The nectary proteome contained 339 identifiable proteins, 71% of which were descriptively annotatable by homology to Plantae. The abundance of 45 proteins differs significantly between male and female nectaries. GO slim analysis revealed a high abundance of proteins related to transport, protein metabolism, carbohydrate metabolism, response to stress, and amino acid metabolism. Comparisons of relative protein abundance revealed that 45 proteins displayed differential expression between male and female nectaries (p-value <0.05); 20 of these proteins were more abundant in male nectaries and 25 were more abundant in female nectaries. GO enrichment of nectary proteins with increased female abundances indicate that female-enriched GO terms are associated with proteins functioning as plasma membrane proton pumps and central metabolism. Proteins associated with male nectary-enriched GO terms were related to cinnamic acid biosynthesis and neutralization of superoxide radicals and hydrogen peroxide.
HostingRepository	PRIDE
AnnounceDate	2018-07-03
AnnouncementXML	Submission_2018-07-17_08:18:30.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009810
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Elizabeth Chatt
SpeciesList	scientific name: Cucurbita maxima; NCBI TaxID: 3661;
ModificationList	Methylthio; Deamidated; Oxidation; iTRAQ8plex; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-17 03:30:45	ID requested
1	2018-07-03 07:50:45	announced
⏵ 2	2018-07-17 08:18:31	announced	Updated publication reference for PubMed record(s): 30008725.

Chatt EC, von Aderkas P, Carter CJ, Smith D, Elliott M, Nikolau BJ, cv. Big Max) Nectar and Nectaries as Determined by Proteomics and Metabolomics. Front Plant Sci, 9():860(2018) [pubmed]

curator keyword: Biological

submitter keyword: nectary, nectar, Cucurbita, pumpkin, floral sex

Dr. Basil J. Nikolau
contact affiliation	Department of Biochemistry Biophysics and Molecular Biology, Iowa State University, Ames, IA, USA
contact email	dimmas@iastate.edu
lab head
Elizabeth Chatt
contact affiliation	Iowa State University
contact email	ecchatt@iastate.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009810
     1. Label: PRIDE project
     2. Name: Sex-dependent variation of pumpkin (Cucurbita maxima cv Big Max) nectar and nectaries as determined by proteomics and metabolomics