PXD009809

PXD009809 is an original dataset announced via ProteomeXchange.

Title	Detailed map of the Pseudomonas aeruginosa Crc regulon using a new parameter for tracking post-transcriptional regulation
Description	Bacterial physiology in general and the bacterial response to the presence of nutrients and to external signals in particular is regulated at different levels, from mRNA synthesis to translational regulation and protein modification. However, there are not standardized parameters for defining post-transcriptional regulation. Herein, we propose a simple parameter, dubbed post-transcriptional variation (PTV), that allow extracting information on translational regulation from the combined analysis of transcriptomic and proteomic data. We have applied this parameter for precisely defining the regulon of the Pseudomonas aeruginosa post-transcriptional regulator Crc. P. aeruginosa is a free-living microorganism that, besides colonizing a diversity of environmental habitats, is able to infect a large number of patients at hospitals. Part of the ability of P. aeruginosa for colonizing such a variety of habitats relies on its capability of using a large number of carbon sources. This hierarchical assimilation is regulated by Crc, which together with Hfq, binds its target RNA impeding their translation when catabolite repression is triggered. Most studies cannot provide information on post-transcriptional regulation when analysed independently. Using the defined PTV parameter, we present a comprehensive map of the Crc post-transcriptional regulon.
HostingRepository	PRIDE
AnnounceDate	2018-11-13
AnnouncementXML	Submission_2018-11-22_08:30:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Concha Gil
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	monohydroxylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-17 02:40:26	ID requested
1	2018-11-13 08:00:37	announced
⏵ 2	2018-11-22 08:30:36	announced	Updated publication reference for PubMed record(s): 30429516, 30411469.

Corona F, Mart, í, nez JL, Nikel PI, The global regulator Crc orchestrates the metabolic robustness underlying oxidative stress resistance in Pseudomonas aeruginosa. Environ Microbiol, 21(3):898-912(2019) [pubmed]

Corona F, Reales-Calder, ó, n JA, Gil C, Mart, í, nez JL, The development of a new parameter for tracking post-transcriptional regulation allows the detailed map of the Pseudomonas aeruginosa Crc regulon. Sci Rep, 8(1):16793(2018) [pubmed]

curator keyword: Biological

submitter keyword: Pseudomonas aeruginosa, P. aeruginosa, post-transcriptional regulation

José Luis Martínez
contact affiliation	Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Madrid, Spain.
contact email	cai.proteomica@pas.ucm.es
lab head
Concha Gil
contact affiliation	UMC
contact email	cai.proteomica@pas.ucm.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD009809

PRIDE project URI

• PRIDE
  1. PXD009809
     1. Label: PRIDE project
     2. Name: Detailed map of the Pseudomonas aeruginosa Crc regulon using a new parameter for tracking post-transcriptional regulation