PXD009802

PXD009802 is an original dataset announced via ProteomeXchange.

Title	phytophthora infestans LC/MS/MS
Description	The oomycete potato blight pathogen Phytophthora infestans secretes a diverse set of proteins to manipulate host plant immunity. However, there is limited knowledge about the role(s) of majority of these secreted proteins. Here we subjected extracellular proteins from in vitro cultured hyphae to LC/MS/MS to investigate the cargo of protein secretion pathways in P. infestans, in particular the conventional secretory pathway. Like the apoplastic effector EPIC1 (a cysteine protease inhibitor), we show that secretion of cell wall degrading enzymes and pathogen associated molecular pattern (PAMP)-like proteins is inhibited by brefeldin A (BFA) in vitro and in planta, demonstrating that these proteins are secreted by the conventional, Golgi-mediated pathway. For comparison, an RXLR (Arg-any amino acid-Leu-Arg) effector, Pi22926, was shown to be unconventionally secreted at haustoria, pathogen structures that penetrate host plant cells, and delivered into infected cells to suppress host defences, consistent with previous findings. We further characterised a pectinesterase (PE), a cell wall degrading enzyme, and INF4, a PAMP-like protein. Both were highly expressed early in infection, enhanced P. infestans colonization, and secreted at haustoria. Notably, in P. infestans transformed lines, PE-mRFP accumulated around the base of haustoria while INF4-mRFP was found to envelop the whole haustorium. We propose that the haustorium is a major site for both non-conventional and conventional secretion, and cell wall degrading enzymes and PAMP proteins are secreted at these sites to facilitate pathogenicity in P. infestans.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:10:28.569.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	shumei wang
SpeciesList	scientific name: Phytophthora infestans T30-4; NCBI TaxID: 403677;
ModificationList	carbamoylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-16 07:36:46	ID requested
1	2018-09-03 02:07:20	announced
⏵ 2	2024-10-22 04:10:36	announced	2024-10-22: Updated project metadata.

Wang S, Welsh L, Thorpe P, Whisson SC, Boevink PC, Birch PRJ, Haustorium Is a Site for Secretion of Diverse Classes of Infection-Associated Proteins. mBio, 9(4):(2018) [pubmed]

10.1128/mbio.01216-18;

curator keyword: Biological

submitter keyword: phytophthora infestans culture filtrate

Paul RJ Birch
contact affiliation	Division of Plant Sciences, University of Dundee (at the James HuttonInstitute), Errol Road, Invergowrie, Dundee DD2 5DA, UK
contact email	Paul.Birch@hutton.ac.uk
lab head
shumei wang
contact affiliation	University of Dundee
contact email	smwang@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD009802

PRIDE project URI

• PRIDE
  1. PXD009802
     1. Label: PRIDE project
     2. Name: phytophthora infestans LC/MS/MS