PXD009794

PXD009794 is an original dataset announced via ProteomeXchange.

Title	EBS is a bivalent histone reader that regulates floral phase transition in Arabidopsis
Description	The ability of cells to perceive and translate versatile cues into differential chromatin and transcriptional states is critical for many biological processes1-4. In plants, timely transition to a flowering state is crucial for successful reproduction5-7. EARLY BOLTING IN SHORT DAY (EBS) is a negative transcriptional regulator that prevents premature flowering in Arabidopsis8,9. Here, we revealed that bivalent bromo-adjacent homology (BAH)-plant homeodomain (PHD) reader modules of EBS bind H3K27me3 and H3K4me3, respectively. A subset of EBS-associated genes was co-enriched with H3K4me3, H3K27me3, and the Polycomb repressor complex 2 (PRC2). Interestingly, EBS adopts an auto-inhibition mode to mediate its binding preference switch between H3K27me3 and H3K4me3. This binding balance is critical because disruption of either EBS-H3K27me3 or EBS-H3K4me3 interaction induces EBS-mediated early floral transition. This study identifies a single bivalent chromatin reader capable of recognizing two antagonistic histone marks and reveals a distinct mechanism of interplay between active and repressive chromatin states.The ability of cells to perceive and translate versatile cues into differential chromatin and transcriptional states is critical for many biological processes1-4. In plants, timely transition to a flowering state is crucial for successful reproduction5-7. EARLY BOLTING IN SHORT DAY (EBS) is a negative transcriptional regulator that prevents premature flowering in Arabidopsis8,9. Here, we revealed that bivalent bromo-adjacent homology (BAH)-plant homeodomain (PHD) reader modules of EBS bind H3K27me3 and H3K4me3, respectively. A subset of EBS-associated genes was co-enriched with H3K4me3, H3K27me3, and the Polycomb repressor complex 2 (PRC2). Interestingly, EBS adopts an auto-inhibition mode to mediate its binding preference switch between H3K27me3 and H3K4me3. This binding balance is critical because disruption of either EBS-H3K27me3 or EBS-H3K4me3 interaction induces EBS-mediated early floral transition. This study identifies a single bivalent chromatin reader capable of recognizing two antagonistic histone marks and reveals a distinct mechanism of interplay between active and repressive chromatin states.v
HostingRepository	PRIDE
AnnounceDate	2019-09-09
AnnouncementXML	Submission_2019-09-09_04:13:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mark Scalf
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	carbamoylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-16 03:23:16	ID requested
⏵ 1	2019-09-09 04:13:22	announced

Yang Z, Qian S, Scheid RN, Lu L, Chen X, Liu R, Du X, Lv X, Boersma MD, Scalf M, Smith LM, Denu JM, Du J, Zhong X, EBS is a bivalent histone reader that regulates floral phase transition in Arabidopsis. Nat Genet, 50(9):1247-1253(2018) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis, histone, chromatin

Xuehua Zhong
contact affiliation	Laboratory of Genetics, University of Wisconsin-Madison, Madison, WI, USA Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI, USA
contact email	xuehua.zhong@wisc.edu
lab head
Mark Scalf
contact affiliation	UW-Madison
contact email	mascalf@wisc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009794
     1. Label: PRIDE project
     2. Name: EBS is a bivalent histone reader that regulates floral phase transition in Arabidopsis