PXD009791

PXD009791 is an original dataset announced via ProteomeXchange.

Title	HLA-DM allotypes - Distinct editing functions of natural HLA-DM allotypes impact antigen presentation and CD4+ T cell activation
Description	Major Histocompatibility Complex of Class II (MHCII) antigen presentation is a key event of adaptive immunity. HLA-DM favors the presentation of kinetically stable peptide-MHCII complexes but does not bind peptides itself and exhibits only a low degree of polymorphism. To date, no evidence for altered function of DM due to natural variations has been reported. We define the presence of DM haplotypes in human populations covered by the 1000 Genomes Project and probe their activity in antigen presentation assays. Our results indicate a clear linkage between DMA*0103 and DMB*0107 never reported before. Thehe corresponding heterodimer features a distinct activity profile when compared to the most frequent allotype (DMA*0101-DMB*0101). In vitro experiments demonstrate a broader pH activity profile for the new allotype, explaining its characteristic cellular function. Together, our results suggest that natural variations of DM have important consequences for an individual’s adaptive immune response.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:24.945.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miguel Alvaro
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-16 02:49:04	ID requested
1	2018-10-31 07:06:39	announced
2	2018-12-06 02:32:40	announced	Updated publication reference for PubMed record(s): 30467419.
⏵ 3	2024-10-22 04:45:26	announced	2024-10-22: Updated project metadata.

10.1038/s41423-018-0181-1;

Á, lvaro-Benito M, Morrison E, Ebner F, Abualrous ET, Urbicht M, Wieczorek M, Freund C, T cell activation. Cell Mol Immunol, 17(2):133-142(2020) [pubmed]

curator keyword: Biological

submitter keyword: Antigen presentation/ MHC class II/ Immunopeptidome/ Peptide editing/ Polymorphism

Christian Freund
contact affiliation	Laboratory of Protein Biochemistry. Department of Biology, Chemistry and Pharmacy. Freie Universität Berlin, Thielallee 63, 14195 Berlin
contact email	chfreund@fu-berlin.de
lab head
Miguel Alvaro
contact affiliation	FU Berlin
contact email	malvaro@zedat.fu-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009791
     1. Label: PRIDE project
     2. Name: HLA-DM allotypes - Distinct editing functions of natural HLA-DM allotypes impact antigen presentation and CD4+ T cell activation