PXD009790

PXD009790 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Human chromatin platforms for the study of transcription and RNA regulation
Description	Here we describe tools to study the step-wise assembly of protein complexes on chromatin in a highly-controlled manner using reconstituted chromatin platforms and quantitative proteomic profiling. We profile the early steps in transcriptional activation and highlight the potential for understanding the multiple ways chromatin can influence transcriptional regulation. We also describe modifications of this approach to study the activity of a long noncoding RNA to act as a dynamic scaffold for proteins to be recruited to chromatin. The reconstituted nature of the chromatin substrate offers a tune-able system, able to be trapped at specific sub-steps, to understand how chromatin interfaces with genome regulation machinery.
HostingRepository	MassIVE
AnnounceDate	2018-09-18
AnnouncementXML	Submission_2018-09-18_19:06:05.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	kirk hansen
SpeciesList	scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932;
ModificationList	Label:13C(6)15N(2); Label:13C(6)15N(4); Label:13C(6); Oxidation; Carboxymethyl; Gln->pyro-Glu; Acetyl
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-15 21:11:25	ID requested
⏵ 1	2018-09-18 19:06:06	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: SILAC chromatin RNA transcription

submitter keyword: SILAC chromatin RNA transcription

Contact List

Aaron Johnson
contact affiliation	University of Colorado Denver
contact email	aaron.m.johnson@ucdenver.edu
lab head
kirk hansen
contact affiliation	university of Colorado
contact email	kirk.hansen@ucdenver.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000082363