<<< Full experiment listing

PXD009789

PXD009789 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSystem-wide profiling of protein-RNA interactions in sindbis infected cells reveals host regulators of infection
DescriptionRNA viruses rely on cellular RNA-binding proteins (RBPs) to infect the host cell. However, the repertoire of the cellular RBPs exploited by viruses is largely unknown. Using the ‘RNA interactome capture’ method, we profiled in a proteome-wide scale the RBP-RNA interactions occurring during sindbis virus (SINV) infection. We discover that SINV affects the activity of hundreds of RBPs, essentially rewiring the host RNA-bound proteome. Such alterations do not relate to changes in protein abundance but are rather due to subcellular redistribution of RBPs and pervasive transcriptome remodelling. Strikingly, RBPs stimulated by SINV accumulate in the cytoplasmic compartments where the virus replicates and interact with viral RNA. Perturbation of these RBPs can restrict or promote infection; for example, GEMIN5 binds to key regulatory regions of SINV RNAs and inhibits viral protein expression. Importantly, we show that drug based RBP intervention may have potential as therapeutic strategy against viruses.
HostingRepositoryPRIDE
AnnounceDate2019-03-01
AnnouncementXMLSubmission_2019-03-01_00:30:32.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCaroline Lenz
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Sindbis virus; NCBI TaxID: 11034;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-05-15 08:56:55ID requested
12019-02-28 09:15:27announced
22019-03-01 00:30:33announcedUpdated project metadata.
Publication List
Garcia-Moreno M, Noerenberg M, Ni S, Järvelin AI, González-Almela E, Lenz CE, Bach-Pages M, Cox V, Avolio R, Davis T, Hester S, Sohier TJM, Li B, Heikel G, Michlewski G, Sanz MA, Carrasco L, Ricci EP, Pelechano V, Davis I, Fischer B, Mohammed S, Castello A, System-wide Profiling of RNA-Binding Proteins Uncovers Key Regulators of Virus Infection. Mol Cell, 74(1):196-211.e11(2019) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Human, Sindbis virus, LC-MSMS
Contact List
Alfredo Castello Palomares
contact affiliationDepartment of Biochemistry, University of Oxford, UK
contact emailalfredo.castellopalomares@bioch.ox.ac.uk
lab head
Caroline Lenz
contact affiliationDepartment of Biochemistry, University of Oxford
contact emailcaroline.lenz@bioch.ox.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD009789
PRIDE project URI
Repository Record List
[ + ]