PXD009767

PXD009767 is an original dataset announced via ProteomeXchange.

Title	Are there enzymatic treatment options for ocular biofilm-based infections?
Description	Pseudomonas aeruginosa-induced corneal keratitis is a sight-threatening disease. The elevated rise of antibiotic resistance among the Pseudomonas keratitis isolates makes treatment of this disease challenging, justifying the need for alternative therapeutic modalities or treatment strategies that can reinforce antibiotic activities. By comparing the responses of P. aeruginosa infection on an outbred strain of mice (SW) to a susceptible strain of mice (C57BL6/N), we found that the neutrophil killing abilities of these strains segregate with their susceptibilities to infection. Namely, SW-derived neutrophils were significantly more efficient at killing P. aeruginosa in vitro than C57BL6/N-derived neutrophils. Quantitative LC-MS/MS analysis revealed significant differences in proteins with enzymatic activities such as alpha mannosidases. Given that during infection, P. aeruginosa forms mannose-rich biofilms at the ocular surface in the susceptible strain of mice, we compared the therapeutic potency of MoAb, recognizing the mannose-rich extracellular polysaccharide, psl, to that of alpha mannosidase. The topical application of alpha mannosidase reduced in vitro-formed biofilms by P. aeruginosa and consistently reduced the bacterial burden of the infected corneas. Cumulatively, these data illustrate that topical application of enzymes can control bacterial biofilm assembly and improve bacterial clearance.
HostingRepository	PRIDE
AnnounceDate	2019-05-15
AnnouncementXML	Submission_2019-05-15_00:56:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jennifer Geddes-McAlister
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	amidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-14 02:29:17	ID requested
⏵ 1	2019-05-15 00:56:47	announced

Kugadas A, Geddes-McAlister J, Guy E, DiGiandomenico A, Sykes DB, Mansour MK, Mirchev R, Gadjeva M, Frontline Science: Employing enzymatic treatment options for management of ocular biofilm-based infections. J Leukoc Biol, 105(6):1099-1110(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Keratitis, neutrophils, P. aeruginosa, biofilms, enzymatic treatment

Jennifer Geddes-McAlister
contact affiliation	Deparment of Molecular and Cellular Biology University of Guelph Guelph, Ontario, Canada Department of Proteomics and Signal Transduction Max Planck Institute of Biochemistry Martinsried, Germany
contact email	jgeddesm@uoguelph.ca
lab head
Jennifer Geddes-McAlister
contact affiliation	University of Guelph
contact email	jgeddesm@uoguelph.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD009767

PRIDE project URI

• PRIDE
  1. PXD009767
     1. Label: PRIDE project
     2. Name: Are there enzymatic treatment options for ocular biofilm-based infections?