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PXD009767

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-05-15
  • AnnouncementXML: Submission_2019-05-15_00:56:46.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Jennifer Geddes-McAlister
  • Title: Are there enzymatic treatment options for ocular biofilm-based infections?
  • Description: Pseudomonas aeruginosa-induced corneal keratitis is a sight-threatening disease. The elevated rise of antibiotic resistance among the Pseudomonas keratitis isolates makes treatment of this disease challenging, justifying the need for alternative therapeutic modalities or treatment strategies that can reinforce antibiotic activities. By comparing the responses of P. aeruginosa infection on an outbred strain of mice (SW) to a susceptible strain of mice (C57BL6/N), we found that the neutrophil killing abilities of these strains segregate with their susceptibilities to infection. Namely, SW-derived neutrophils were significantly more efficient at killing P. aeruginosa in vitro than C57BL6/N-derived neutrophils. Quantitative LC-MS/MS analysis revealed significant differences in proteins with enzymatic activities such as alpha mannosidases. Given that during infection, P. aeruginosa forms mannose-rich biofilms at the ocular surface in the susceptible strain of mice, we compared the therapeutic potency of MoAb, recognizing the mannose-rich extracellular polysaccharide, psl, to that of alpha mannosidase. The topical application of alpha mannosidase reduced in vitro-formed biofilms by P. aeruginosa and consistently reduced the bacterial burden of the infected corneas. Cumulatively, these data illustrate that topical application of enzymes can control bacterial biofilm assembly and improve bacterial clearance.
  • SpeciesList: scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
  • ModificationList: amidated residue; iodoacetamide derivatized residue
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-05-14 02:29:17ID requested
12019-05-15 00:56:47announced

Publication List

  1. Kugadas A, Geddes-McAlister J, Guy E, DiGiandomenico A, Sykes DB, Mansour MK, Mirchev R, Gadjeva M, Frontline Science: Employing enzymatic treatment options for management of ocular biofilm-based infections. J Leukoc Biol, 105(6):1099-1110(2019) [pubmed]

Keyword List

  1. curator keyword: Biomedical
  2. submitter keyword: Keratitis, neutrophils, P. aeruginosa, biofilms, enzymatic treatment

Contact List

    Jennifer Geddes-McAlister
    • contact affiliation: Deparment of Molecular and Cellular Biology University of Guelph Guelph, Ontario, Canada Department of Proteomics and Signal Transduction Max Planck Institute of Biochemistry Martinsried, Germany
    • contact email: jgeddesm@uoguelph.ca
    • lab head:
    Jennifer Geddes-McAlister
    • contact affiliation: University of Guelph
    • contact email: jgeddesm@uoguelph.ca
    • dataset submitter:

Full Dataset Link List

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