PXD009747

PXD009747 is an original dataset announced via ProteomeXchange.

Title	Centrosomal ALIX regulates mitotic spindle orientation by modulating astral microtubule dynamics
Description	The orientation of the mitotic spindle (MS) is tightly regulated, but the molecular mechanisms are incompletely understood. Here we report a novel role for the multifunctional adaptor protein ALG-2-interacting protein X (ALIX) in regulating MS orientation in addition to its well-established role in cytokinesis. We show that ALIX is recruited to the pericentriolar material (PCM) of the centrosomes and promotes correct orientation of the MS in asymmetrically dividing Drosophila stem cells and epithelial cells, and symmetrically dividing Drosophila and human epithelial cells. ALIX-deprived cells display defective formation of astral microtubules (MTs), which results in abnormal MS orientation. Specifically, ALIX is recruited to the PCM via Drosophila Spindle defective 2 (DSpd-2)/Cep192, where ALIX promotes accumulation of g-tubulin and thus facilitates efficient nucleation of astral MTs. In addition, ALIX promotes MT stability by recruiting Microtubule Associated Protein 1S (MAP1S), which stabilizes newly formed MTs. Altogether, our results demonstrate a novel evolutionarily conserved role of ALIX in providing robustness to the orientation of the MS by promoting astral MT formation during asymmetric and symmetric cell division.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:23.827.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tuula Nyman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-11 01:07:25	ID requested
1	2018-06-04 06:35:21	announced
2	2019-06-14 07:41:38	announced	Updated publication reference for PubMed record(s): 29858227.
⏵ 3	2024-10-22 04:45:33	announced	2024-10-22: Updated project metadata.

10.15252/embj.201797741;

Maler, ø, d L, Le Borgne R, Lie-Jensen A, Eikenes Å H, Brech A, Liest, ø, l K, Stenmark H, Haglund K, Centrosomal ALIX regulates mitotic spindle orientation by modulating astral microtubule dynamics. EMBO J, 37(13):(2018) [pubmed]

curator keyword: Biological

submitter keyword: mitotic spindle orientation,ALIX, microtubule, g-tubulin, MAP1S

Tuula Nyman
contact affiliation	Head of Proteomics
contact email	tuula.nyman@medisin.uio.no
lab head
Tuula Nyman
contact affiliation	University of Oslo
contact email	tuula.nyman@medisin.uio.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD009747

PRIDE project URI

• PRIDE
  1. PXD009747
     1. Label: PRIDE project
     2. Name: Centrosomal ALIX regulates mitotic spindle orientation by modulating astral microtubule dynamics