PXD009746

PXD009746 is an original dataset announced via ProteomeXchange.

Title	Label-free Proteomic Analysis of Exosomes Secreted from THP-1-Derived Macrophages in the Absence or Presence of Interferon-α
Description	Exosomes are extracellular vesicles that function in intercellular communication. We have previously reported that exosomes play a role in the transmission of antiviral molecules during interferon-α (IFN-α)-mediated immune responses. In this study, the protein contents of THP-1-derived macrophages with or without interferon-α treatment and of the exosomes secreted from these cells were analyzed by the label-free LC-MS/MS quantitation technologies. A total number of 1845 and 1550 protein groups were identified in the THP-1 macrophages and the corresponding exosomes, respectively. Treating the cells with IFN-α resulted in the differential abundance of 110 proteins in cells and 260 proteins in exosomes (greater than 2.0-fold), among which 35 proteins were both up-regulated in the IFN-α treated cells and corresponding exosomes while 139 proteins were specifically up-regulated in exosomes but not in the donor cells. GO and KEGG analysis of the protein function categories suggested that IFN-α promoted the abundance of proteins involved in “defense response to virus” in both exosomes and cells, and proteins related to “RNA processing” only in exosomes. Functional analysis further indicated that exosomes from IFN-α-treated cells exhibited potent antiviral activity that restored the impaired antiviral response of IFN-α in hepatitis B virus-replicating hepatocytes. These results have deepened the understanding of the exosome-mediated transfer of IFN-α-induced antiviral molecules and may provide new basis for therapeutic strategies to control viral infection.
HostingRepository	PRIDE
AnnounceDate	2018-12-17
AnnouncementXML	Submission_2018-12-21_01:28:40.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009746
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dominik Andre Meger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Propionamide; Oxidation; residues isobaric at 71.037114 Da
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-11 00:51:45	ID requested
1	2018-12-17 04:27:48	announced
⏵ 2	2018-12-21 01:28:42	announced	Updated publication reference for PubMed record(s): 30550287.

Yao Z, Jia X, Megger DA, Chen J, Liu Y, Li J, Sitek B, Yuan Z, Identifies Antiviral Proteins Enriched in Exosomes. J Proteome Res, 18(3):855-864(2019) [pubmed]

curator keyword: Biological

submitter keyword: IFN-α

Exosomes

Macrophages

Label-free quantification

ISGs

Dominik Andre Megger
contact affiliation	Medizinisches Proteom-Center Ruhr-Universität Bochum 44801 Bochum Germany
contact email	dominik.megger@rub.de
lab head
Dominik Andre Meger
contact affiliation	Medizinisches Proteom-Center (MPC), Ruhr-Universität Bochum, Germany
contact email	dominik.megger@rub.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009746
     1. Label: PRIDE project
     2. Name: Label-free Proteomic Analysis of Exosomes Secreted from THP-1-Derived Macrophages in the Absence or Presence of Interferon-α