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PXD009745

PXD009745 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe activity-dependent bulk endocytosis proteome reveals a key presynaptic role for Rab11
DescriptionActivity-dependent bulk endocytosis (ADBE) is the dominant mode of synaptic vesicle (SV) endocytosis during high frequency stimulation, suggesting it should play key roles in neurotransmission during periods of intense neuronal activity. However efforts in elucidating the physiological role of ADBE have been hampered by the lack of identified molecules which are unique to this endocytosis mode. To address this, we performed proteomic analysis on purified bulk endosomes, which are a key intermediate in ADBE. Bulk endosomes were enriched via two independent approaches, a classical subcellular fractionation method and isolation via magnetic nanoparticles. There was a 79 % overlap in proteins identified via the two protocols and these molecules formed the ADBE proteome. Bioinformatic analysis revealed a strong enrichment in cell adhesion, cytoskeletal and signalling molecules, in addition to expected SV and trafficking proteins. Network analysis identified rab GTPases as a central hub within the ADBE proteome. Subsequent investigation of a subset of these rabs revealed that constitutively active rab11 both facilitated ADBE and accelerated clathrin-mediated endocytosis. This first result suggests that the ADBE proteome will provide a rich resource for the future study of presynaptic function.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:45:02.972.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMatthias Trost
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListdeaminated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-05-11 00:22:47ID requested
12018-10-15 15:18:00announced
22024-10-22 04:45:03announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: Dimethyl, Quantitative proteomics, nycozend and magnetic nanoparticles, endosomes
Contact List
Matthias Trost
contact affiliationInstitute for Cell and molecular Biosciences (ICAMB) Faculty of medical Sciences, Newcastle University Framlington place Newcastle Upon Tyne, NE2 4HH, UK
contact emailmatthias.trost@ncl.ac.uk
lab head
Matthias Trost
contact affiliationNewcastle University
contact emailmatthias.trost@ncl.ac.uk
dataset submitter
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Dataset FTP location
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