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PXD009732

PXD009732 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNative mass spectrometry of ClpB
DescriptionThe ATP-dependent bacterial protein disaggregation machine, ClpB belonging to the AAA+ superfamily, refolds toxic protein aggregates into the native state in cooperation with the cognate Hsp70 partner. The ring-shaped hexamers of ClpB unfold and thread its protein substrate through the central pore. However, their function-related structural dynamics has remained elusive. Here we directly visualize ClpB using high-speed atomic force microscopy (HS-AFM) to gain a mechanistic insight into its disaggregation function. The HS-AFM movies demonstrate massive conformational changes of the hexameric ring during ATP hydrolysis, from a round ring to a spiral and even to a pair of twisted half-spirals. HS-AFM observations of Walker-motif mutants unveil crucial roles of ATP binding and hydrolysis in the oligomer formation and structural dynamics. Furthermore, repressed and hyperactive mutations result in significantly different oligomeric forms. These results provide a comprehensive view for the ATP-driven oligomeric-state transitions that enable ClpB to disentangle protein aggregates
HostingRepositoryPRIDE
AnnounceDate2018-10-18
AnnouncementXMLSubmission_2018-10-18_03:08:21.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKentaro Ishii
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListNo PTMs are included in the dataset
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-05-09 09:34:18ID requested
12018-10-18 03:08:23announced
Publication List
Uchihashi T, Watanabe YH, Nakazaki Y, Yamasaki T, Watanabe H, Maruno T, Ishii K, Uchiyama S, Song C, Murata K, Iino R, Ando T, Dynamic structural states of ClpB involved in its disaggregation function. Nat Commun, 9(1):2147(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Native mass spectrometry, ClpB
Contact List
Susumu Uchiyama
contact affiliation5Department of Biotechnology, Graduate School of Engineering, Osaka University
contact emailsuchi@bio.eng.osaka-u.ac.jp
lab head
Kentaro Ishii
contact affiliationOkazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences
contact emailishii@ims.ac.jp
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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