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PXD009714

PXD009714 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
DescriptionHerein, we describe a method, dubbed PROMIS that allows simultaneous, global analysis of endogenous protein–small-molecule and protein-protein complexes. To this end a cell-free native lysate was subjected to size-exclusion chromatography followed by quantitative metabolomic and proteomic analysis. Applying this approach to an extract from Arabidopsis thaliana cell cultures, we could retrieve known protein-protein (PPI) and protein–metabolite (PMI) interactions, validating our strategy.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:45:09.576.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMichal Gorka
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-05-08 06:52:39ID requested
12018-06-06 02:39:52announced
22024-10-22 04:45:20announced2024-10-22: Updated project metadata.
Publication List
Veyel D, Sokolowska EM, Moreno JC, Kierszniowska S, Cichon J, Wojciechowska I, Luzarowski M, Kosmacz M, Szlachetko J, Gorka M, M, é, ret M, Graf A, Meyer EH, Willmitzer L, Skirycz A, . J Biol Chem, 293(32):12440-12453(2018) [pubmed]
10.1074/jbc.ra118.003351;
Keyword List
curator keyword: Technical, Biological
submitter keyword: protein-metabolite, size exclusion chromatografy, deconvolution, correlation,Interactions
Contact List
Aleksandra Skirycz
contact affiliationMax Planck Institute for Molecular Plant Physiology, Small Molecule Signaling Department
contact emailskirycz@mpimp-golm.mpg.de
lab head
Michal Gorka
contact affiliationMax Planck Institute for Molecular Plant Physiology | Celon Pharma
contact emailgorka@mpimp-golm.mpg.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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