PXD009714

PXD009714 is an original dataset announced via ProteomeXchange.

Title	PROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
Description	Herein, we describe a method, dubbed PROMIS that allows simultaneous, global analysis of endogenous protein–small-molecule and protein-protein complexes. To this end a cell-free native lysate was subjected to size-exclusion chromatography followed by quantitative metabolomic and proteomic analysis. Applying this approach to an extract from Arabidopsis thaliana cell cultures, we could retrieve known protein-protein (PPI) and protein–metabolite (PMI) interactions, validating our strategy.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:09.576.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michal Gorka
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-08 06:52:39	ID requested
1	2018-06-06 02:39:52	announced
⏵ 2	2024-10-22 04:45:20	announced	2024-10-22: Updated project metadata.

Veyel D, Sokolowska EM, Moreno JC, Kierszniowska S, Cichon J, Wojciechowska I, Luzarowski M, Kosmacz M, Szlachetko J, Gorka M, M, é, ret M, Graf A, Meyer EH, Willmitzer L, Skirycz A, . J Biol Chem, 293(32):12440-12453(2018) [pubmed]

10.1074/jbc.ra118.003351;

curator keyword: Technical, Biological

submitter keyword: protein-metabolite, size exclusion chromatografy, deconvolution, correlation,Interactions

Aleksandra Skirycz
contact affiliation	Max Planck Institute for Molecular Plant Physiology, Small Molecule Signaling Department
contact email	skirycz@mpimp-golm.mpg.de
lab head
Michal Gorka
contact affiliation	Max Planck Institute for Molecular Plant Physiology | Celon Pharma
contact email	gorka@mpimp-golm.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD009714

PRIDE project URI

• PRIDE
  1. PXD009714
     1. Label: PRIDE project
     2. Name: PROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana