PXD009709

PXD009709 is an original dataset announced via ProteomeXchange.

Title	Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine
Description	Extracellular vesicles (EVs) have a great potential in clinical applications, however their isolation from different body fluids and their characterisation are not yet optimal or standardised. Here we report the results of examining the performance of ultrafiltration combined with size exclusion chromatography (UF-SEC) to isolate EVs from urine. The results reveal that UF-SEC is a fast method and provides high purity. Furthermore, we introduce asymmetrical-flow field-flow fractionation coupled with a UV detector and multi-angle light-scattering detector (AF4/UV-MALS) as a characterisation method and compare it with the applicabilities and limitations of current methods. We demonstrate that AF4/UV-MALS is a straightforward characterisation method for urinary EVs.
HostingRepository	PRIDE
AnnounceDate	2018-07-04
AnnouncementXML	Submission_2019-06-17_03:30:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kurt Boonen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-08 02:38:43	ID requested
1	2018-07-04 07:27:06	announced
⏵ 2	2019-06-17 03:30:29	announced	Updated publication reference for PubMed record(s): 29988836.

Oeyen E, Van Mol K, Baggerman G, Willems H, Boonen K, Rolfo C, Pauwels P, Jacobs A, Schildermans K, Cho WC, Mertens I, Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine. J Extracell Vesicles, 7(1):1490143(2018) [pubmed]

curator keyword: Technical, Biological

submitter keyword: lc-ms/ms, urine, extracellular vesicle, exosome, orbitrap

Inge Mertens
contact affiliation	Flemish Institute for Technological Research (VITO) Boeretang 200 2400 Mol
contact email	inge.mertens@vito.be
lab head
Kurt Boonen
contact affiliation	University of Antwerp - VITO
contact email	kurt.boonen@vito.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD009709

PRIDE project URI

• PRIDE
  1. PXD009709
     1. Label: PRIDE project
     2. Name: Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine