PXD009683

PXD009683 is an original dataset announced via ProteomeXchange.

Title	Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells
Description	Rab GTPases recruit peripheral membrane proteins and can define organelle identity. Rab18 localizes to the ER but also to the surfaces of lipid droplets (LDs), where it has been implicated in effector protein recruitment and in defining LD identity. Here, we studied Rab18 localization and function in SUM159 cells. Rab18 localized to the ER and to LD membranes upon LD induction, with the latter depending on the Rab18 activation state. In cells lacking Rab18, LDs were modestly reduced in size and numbers, but we found little evidence for Rab18 function in LD formation, LD turnover upon cell starvation, or the targeting of several proteins to LDs. We conclude that Rab18 is not a general, necessary component of the protein machinery involved in LD biogenesis or turnover, but instead likely plays a specialized role in specific cell types
HostingRepository	PRIDE
AnnounceDate	2018-06-06
AnnouncementXML	Submission_2018-06-06_05:34:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zon Lai
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-04 09:00:39	ID requested
⏵ 1	2018-06-06 05:34:27	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Lipid droplet, Rab18, RabGTPase, Endoplasmic reticulum, Triacylglycerol, Neutral lipid storage

Tobias Walther
contact affiliation	Harvard University
contact email	twalther@hsph.harvard.edu
lab head
Zon Lai
contact affiliation	Harvard T. H. Chan School of Public Health
contact email	zlai@hsph.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009683
     1. Label: PRIDE project
     2. Name: Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells