PXD009678

PXD009678 is an original dataset announced via ProteomeXchange.

Title	Multi-omics metabolism analysis on irradiation-induced oxidative stress to Rhodotorula glutinis
Description	Oxidative stress is induced by various natural abiotic factors including irradiation among many microorganisms and has been demonstrated that it significantly improved growth rate and lipid production of R. glutinis. However, the specific mechanisms of how irradiation influence the metabolism of R. glutinis remains still unavailable. To investigate and better understand the mechanisms involved in irradiation-induced stress resistance in R. glutinis, a multi-omics metabolism analysis was implemented. The results confirmed that irradiation indeed not only improve cell biomass but also accelerate carotenoids and lipid production, especially neutral lipid. Compared with the control, metabolome profiling in the group exposed to irradiation exhibited obvious difference in the activation of TCA cycle and transition of glucose utilization pattern from glycolysis to pentose phosphate pathway. The results of proteome showed that 423 proteins were changed significantly and proteins associated with protein folding and transport, the Hsp40 and Sec12, were obviously up-regulated, indicating that cells responded to irradiation by accelerating the protein folding and transport of correctly folded proteins as well as enhanced the degradation of misfolded proteins. A significant up-regulation of carotenoids biosynthetic pathway was observed which revealed that increased carotenoids is a cellular defense mechanism against oxidative stress generated by irradiation. Therefore, the results of comprehensive omics analysis provide new insights on the response and tolerance mechanism of R. glutinis to irradiation-induced oxidative stress resistance and could be helpful in the further investigation of R. glutinis as model microorganism for biofuel production.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:56.857.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guiping Gong
SpeciesList	scientific name: Rhodotorula glutinis; NCBI TaxID: 5535;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-04 07:06:56	ID requested
1	2018-10-25 08:55:07	announced
⏵ 2	2024-10-22 04:45:02	announced	2024-10-22: Updated project metadata.

10.1007/s00253-018-9448-9;

Gong G, Liu L, Zhang X, Tan T, Multi-omics metabolism analysis on irradiation-induced oxidative stress to Rhodotorula glutinis. Appl Microbiol Biotechnol, 103(1):361-374(2019) [pubmed]

curator keyword: Biological

submitter keyword: Rhodotorula glutinis

irradiation

stress response

multi-omics analysis

Guiping Gong
contact affiliation	Beijing Key Lab of Bioprocess, National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China
contact email	18810263854@163.com
lab head
Guiping Gong
contact affiliation	Beijing University of Chemical Technology
contact email	18810263854@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009678
     1. Label: PRIDE project
     2. Name: Multi-omics metabolism analysis on irradiation-induced oxidative stress to Rhodotorula glutinis