PXD009657

PXD009657 is an original dataset announced via ProteomeXchange.

Title	In-situ Click Reaction Coupled with Quantitative Proteomics for Identification of Protein Targets of Catechol Estrogens
Description	Catechol estrogens (CEs) are metabolic electrophiles that actively undergo covalent interaction with cellular proteins, influencing molecular function. There is no feasible method to identify their binders in a living system. Herein, we developed a click chemistry-based approach using ethinylestradiol(EE2) as the precursor probe coupled with quantitative proteomics to identify protein targets of CEs and classify their binding strengths.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:23.660.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi-Chen Liu
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-03 01:04:41	ID requested
1	2022-02-28 03:01:06	announced
⏵ 2	2023-11-14 08:49:24	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Technical

submitter keyword: post translational modification, metabolites, proteomics, electrophiles, dimethyl labeling, LC/MS,catechol estrogens, oxidative stress, click chemistry, redox modification

Chen, Shu-Hui
contact affiliation	Department of Chemistry,Department of Life Sciences,National Cheng Kung University, Tainan, Taiwan
contact email	shchen@mail.ncku.edu.tw
lab head
Yi-Chen Liu
contact affiliation	Department of Chemistry, National Cheng Kung University, Tainan, Taiwan
contact email	yichenliu2020@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD009657

PRIDE project URI

• PRIDE
  1. PXD009657
     1. Label: PRIDE project
     2. Name: In-situ Click Reaction Coupled with Quantitative Proteomics for Identification of Protein Targets of Catechol Estrogens