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PXD009651

PXD009651 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHiRIEF TMT Proteomics of Arabidosis thaliana, wildtype versus oligopeptidases' KO mutants
DescriptionOligopeptidases Prep and OOP are enzymes located in mitochondria and chloroplasts responsible for the cleavage of long peptides (8 to 65 aas) down to short peptides (3 to 7 aas), which in turn are degraded to free amino acids by aminopeptidases such as M1 and M17. In this study, Arabidopsis thaliana knockout mutants of the genes for these enzymes were studied by proteomics using a TMT (tandem mass tags) isobaric tags quantification strategy combined with HiRIEF LC-MS on a Q-Exactive Mass Spectrometer.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:44:45.222.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRui Branca
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-05-02 10:53:42ID requested
12018-11-14 03:23:15announced
22024-10-22 04:44:51announced2024-10-22: Updated project metadata.
Publication List
10.1111/tpj.14100;
Kmiec B, Branca RMM, Berkowitz O, Li L, Wang Y, Murcha MW, Whelan J, Lehti, ö J, Glaser E, Teixeira PF, Accumulation of endogenous peptides triggers a pathogen stress response in Arabidopsis thaliana. Plant J, 96(4):705-715(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Arabidopsis thaliana, proteomics, TMT, PreP, oligopeptidase, HiRIEF, LCMS, OOP
Contact List
Janne Lehtiö
contact affiliationDept. Oncology-Pathology, Science for Life Laboratory, Karolinska Institutet, Sweden
contact emailjanne.lehtio@ki.se
lab head
Rui Branca
contact affiliationClinical Proteomics Unit, Dep. of Oncology-Pathology
contact emailrui.mamede-branca@ki.se
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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