PXD009651

PXD009651 is an original dataset announced via ProteomeXchange.

Title	HiRIEF TMT Proteomics of Arabidosis thaliana, wildtype versus oligopeptidases' KO mutants
Description	Oligopeptidases Prep and OOP are enzymes located in mitochondria and chloroplasts responsible for the cleavage of long peptides (8 to 65 aas) down to short peptides (3 to 7 aas), which in turn are degraded to free amino acids by aminopeptidases such as M1 and M17. In this study, Arabidopsis thaliana knockout mutants of the genes for these enzymes were studied by proteomics using a TMT (tandem mass tags) isobaric tags quantification strategy combined with HiRIEF LC-MS on a Q-Exactive Mass Spectrometer.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:45.222.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rui Branca
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-02 10:53:42	ID requested
1	2018-11-14 03:23:15	announced
⏵ 2	2024-10-22 04:44:51	announced	2024-10-22: Updated project metadata.

10.1111/tpj.14100;

Kmiec B, Branca RMM, Berkowitz O, Li L, Wang Y, Murcha MW, Whelan J, Lehti, ö J, Glaser E, Teixeira PF, Accumulation of endogenous peptides triggers a pathogen stress response in Arabidopsis thaliana. Plant J, 96(4):705-715(2018) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis thaliana, proteomics, TMT, PreP, oligopeptidase, HiRIEF, LCMS, OOP

Janne Lehtiö
contact affiliation	Dept. Oncology-Pathology, Science for Life Laboratory, Karolinska Institutet, Sweden
contact email	janne.lehtio@ki.se
lab head
Rui Branca
contact affiliation	Clinical Proteomics Unit, Dep. of Oncology-Pathology
contact email	rui.mamede-branca@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD009651

PRIDE project URI

• PRIDE
  1. PXD009651
     1. Label: PRIDE project
     2. Name: HiRIEF TMT Proteomics of Arabidosis thaliana, wildtype versus oligopeptidases' KO mutants