PXD009651 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | HiRIEF TMT Proteomics of Arabidosis thaliana, wildtype versus oligopeptidases' KO mutants |
Description | Oligopeptidases Prep and OOP are enzymes located in mitochondria and chloroplasts responsible for the cleavage of long peptides (8 to 65 aas) down to short peptides (3 to 7 aas), which in turn are degraded to free amino acids by aminopeptidases such as M1 and M17. In this study, Arabidopsis thaliana knockout mutants of the genes for these enzymes were studied by proteomics using a TMT (tandem mass tags) isobaric tags quantification strategy combined with HiRIEF LC-MS on a Q-Exactive Mass Spectrometer. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:44:45.222.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rui Branca |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-05-02 10:53:42 | ID requested | |
1 | 2018-11-14 03:23:15 | announced | |
⏵ 2 | 2024-10-22 04:44:51 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1111/tpj.14100; |
Kmiec B, Branca RMM, Berkowitz O, Li L, Wang Y, Murcha MW, Whelan J, Lehti, ö J, Glaser E, Teixeira PF, Accumulation of endogenous peptides triggers a pathogen stress response in Arabidopsis thaliana. Plant J, 96(4):705-715(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Arabidopsis thaliana, proteomics, TMT, PreP, oligopeptidase, HiRIEF, LCMS, OOP |
Contact List
Janne Lehtiö |
contact affiliation | Dept. Oncology-Pathology, Science for Life Laboratory, Karolinska Institutet, Sweden |
contact email | janne.lehtio@ki.se |
lab head | |
Rui Branca |
contact affiliation | Clinical Proteomics Unit, Dep. of Oncology-Pathology |
contact email | rui.mamede-branca@ki.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009651
- Label: PRIDE project
- Name: HiRIEF TMT Proteomics of Arabidosis thaliana, wildtype versus oligopeptidases' KO mutants