PXD009629

PXD009629 is an original dataset announced via ProteomeXchange.

Title	Photoferrotroph R. palustris TIE-1
Description	The underlying adaptations required by anoxygenic phototrophs to oxidize Fe(II), a potential stressor, are not well constrained. We used quantitative proteomics to compare cells of the photoferrotroph Rhodopseudomonas palustris TIE-1 grown photoautotrophically with Fe(II) or H2, and photoheterotrophically with acetate. We observed unique proteome profiles for each condition with differences primarily driven by carbon source. Growth on Fe(II) was characterized by a response typical of iron homeostasis which included an increased abundance of proteins required for metal efflux (particularly copper), and decreased abundance of iron import proteins, including siderophore receptors, with no evidence of further stressors such as oxidative damage. This study suggests that the main challenge facing photoferrotrophs comes from limitations imposed by autotrophic growth and, once this challenge is overcome, iron stress can be mitigated using iron management mechanisms common to diverse bacteria.
HostingRepository	PRIDE
AnnounceDate	2018-06-25
AnnouncementXML	Submission_2018-06-24_23:57:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicolas Nalpas
SpeciesList	scientific name: Rhodopseudomonas palustris TIE-1; NCBI TaxID: 395960;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-30 03:25:33	ID requested
⏵ 1	2018-06-24 23:57:17	announced

Bryce C, Franz-Wachtel M, Nalpas NC, Miot J, Benzerara K, Byrne JM, Kleindienst S, Macek B, Kappler A, Proteome Response of a Metabolically Flexible Anoxygenic Phototroph to Fe(II) Oxidation. Appl Environ Microbiol, 84(16):(2018) [pubmed]

curator keyword: Biological

submitter keyword: Photoferrotroph, iron, oxidation, anoxygenic photosynthesis

Prof. Dr. Andreas Kappler
contact affiliation	Geomicrobiology Center for Applied Geoscience (ZAG) University of Tübingen
contact email	andreas.kappler@uni-tuebingen.de
lab head
Nicolas Nalpas
contact affiliation	Tuebingen University
contact email	nalpas.nicolas@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD009629

PRIDE project URI

• PRIDE
  1. PXD009629
     1. Label: PRIDE project
     2. Name: Photoferrotroph R. palustris TIE-1