PXD009604

PXD009604 is an original dataset announced via ProteomeXchange.

Title	Toxoplasma GRA protein identification
Description	Toxoplasma gondii is an opportunistic pathogen infecting humans and a variety of vertebrate animals. Secretory dense granule proteins (GRA) are important effectors that mediate host-parasite interactions and facilitate parasitism. Due to the wide host range, T. gondii needs diverse GRA proteins to accommodate parasite survival in different hosts. In this study, we used two proximity based protein labeling techniques to identify novel GRA proteins. Using GRA1 as bait, two transgenic strains expressing GRA1-BirA* or GRA1-APEX were used to biotinylate GRA candidate proteins. Using these methods, 90 proteins were identified by APEX based labeling and 56 by the BirA* approach. Twenty-three were identified by both techniques, among which 13 are known GRA proteins. In combination 98 novel GRA candidates were identified by these two approaches and 25 are known GRAs. The majority of them are only present in coccidian parasites and dispensable for parasite growth in vitro, they likely mediate interactions with hosts and play roles during animal infections.
HostingRepository	PRIDE
AnnounceDate	2018-11-02
AnnouncementXML	Submission_2018-11-02_02:41:24.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009604
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bang Shen
SpeciesList	scientific name: Toxoplasma; NCBI TaxID: 5810;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	Q-Tof ultima

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-25 08:35:23	ID requested
⏵ 1	2018-11-02 02:41:25	announced

Pan M, Li M, Li L, Song Y, Hou L, Zhao J, Shen B, Identification of Novel Dense-Granule Proteins in Toxoplasma gondii by Two Proximity-Based Biotinylation Approaches. J Proteome Res, 18(1):319-330(2019) [pubmed]

curator keyword: Biological

submitter keyword: Toxoplasma, GRA, APEX, BirA

Bang Shen
contact affiliation	State Key laboratory of Agricultural Microbiology, College of Veterinary Medcine, Huazhong Agricultural University, Wuhan, Hubei, PR China
contact email	shenbang@mail.hzau.edu.cn
lab head
Bang Shen
contact affiliation	Huazhong Agricultural University
contact email	shenbang@mail.hzau.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009604
     1. Label: PRIDE project
     2. Name: Toxoplasma GRA protein identification