PXD009603

PXD009603 is an original dataset announced via ProteomeXchange.

Title	Proteomic Evidence of Dietary Sources in Ancient Dental Calculus
Description	Archaeological dental calculus has emerged as a rich source of ancient biomolecules, including proteins. Previous analyses of proteins extracted from ancient dental calculus revealed the presence of the dietary milk protein β-lactoglobulin, providing direct evidence of dairy consumption in the archaeological record. However, the potential for calculus to preserve other food-related proteins has not yet been systematically explored. Here we analyse shotgun metaproteomic data from 100 archaeological dental calculus samples ranging from the Iron Age to the post-medieval period (8thC BC - 19thC AD) in Britain, as well as dental calculus from contemporary dental patients and recently deceased individuals, to characterise the range and extent of dietary proteins preserved in dental calculus. In addition to milk proteins, we detected proteomic evidence of foodstuffs such as cereals and plant products, as well as the digestive enzyme salivary amylase. We discuss the importance of optimized protein extraction methods, data analysis approaches, and authentication strategies in the identification of dietary proteins from archaeological dental calculus. Our ability to detect dietary proteins, although limited, demonstrates the potential of these methods to robustly identify foodstuffs in the archaeological record that are under-represented due to their poor preservation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:09.119.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jessica Hendy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-25 08:27:44	ID requested
1	2018-07-18 03:33:50	announced
2	2018-07-31 02:22:03	announced	Updated publication reference for PubMed record(s): 30051838.
⏵ 3	2024-10-22 04:45:20	announced	2024-10-22: Updated project metadata.

Hendy J, Warinner C, Bouwman A, Collins MJ, Fiddyment S, Fischer R, Hagan R, Hofman CA, Holst M, Chaves E, Klaus L, Larson G, Mackie M, McGrath K, Mundorff AZ, Radini A, Rao H, Trachsel C, Velsko IM, Speller CF, Proteomic evidence of dietary sources in ancient dental calculus. Proc Biol Sci, 285(1883):(2018) [pubmed]

10.1098/rspb.2018.0977;

curator keyword: Biological

submitter keyword: Human, Dental Calculus, Dental Plaque, LC-MS/MS

Jessica Hendy
contact affiliation	Max Planck Institute for the Science of Human History
contact email	hendy@shh.mpg.de
lab head
Jessica Hendy
contact affiliation	Max Planck Institute for the Science of Human History
contact email	hendy@shh.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009603
     1. Label: PRIDE project
     2. Name: Proteomic Evidence of Dietary Sources in Ancient Dental Calculus