PXD009601

PXD009601 is an original dataset announced via ProteomeXchange.

Title	Diverged Trypanosome MICOS as a Hub for Mitochondrial Cristae Shaping and Protein Import
Description	The mitochondrial contact site and cristae organization system (MICOS) is a complex responsible for proper cristae formation. Empirical data about this ubiquitous complex is limited to opisthokont models (e.g. yeast), impeding understanding of its core properties and adaptation potential to diverse cellular milieus. To close this knowledge gap, we characterized MICOS from the diverged excavate Trypanosoma brucei. These results allow the definition of fundamental MICOS properties, such as its role in maintaining both cristae and contacts between the inner and outer membranes plus its extrusion into the intermembrane space. Yet, surprising differences with opisthokonts indicate that MICOS can assume different forms. T. brucei MICOS contains a novel subunit involved in the import of intermembrane space proteins, including respiratory chain complex assembly factors. We propose an evidence-based hypothesis that T. brucei MICOS populates cristae membranes with proteins needed for respiration, supported by the complex's even distribution in cristae
HostingRepository	PRIDE
AnnounceDate	2018-09-13
AnnouncementXML	Submission_2019-06-17_03:24:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Potesil
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-25 08:26:06	ID requested
1	2018-09-13 11:25:44	announced
⏵ 2	2019-06-17 03:24:53	announced	Updated publication reference for PubMed record(s): 30415698.

Kaurov I, Vancov, á M, Schimanski B, Cadena LR, Heller J, B, í, l, ý T, Pot, ě, š, il D, Eichenberger C, Bruce H, Oeljeklaus S, Warscheid B, Zdr, á, hal Z, Schneider A, Luke, š J, Hashimi H, The Diverged Trypanosome MICOS Complex as a Hub for Mitochondrial Cristae Shaping and Protein Import. Curr Biol, 28(21):3393-3407.e5(2018) [pubmed]

curator keyword: Biological

submitter keyword: Mitochondria

cristae

intermembrane space

MICOS

protein import

Trypanosoma brucei

LC-MS/MS

label-free

TMT

Zbyněk Zdráhal
contact affiliation	CEITEC-MU, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
David Potesil
contact affiliation	Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic
contact email	david.potesil@ceitec.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD009601

PRIDE project URI

• PRIDE
  1. PXD009601
     1. Label: PRIDE project
     2. Name: Diverged Trypanosome MICOS as a Hub for Mitochondrial Cristae Shaping and Protein Import