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PXD009601

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2018-09-13
  • AnnouncementXML: Submission_2018-09-13_11:25:43.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: David Potesil
  • Title: Diverged Trypanosome MICOS as a Hub for Mitochondrial Cristae Shaping and Protein Import
  • Description: The mitochondrial contact site and cristae organization system (MICOS) is a complex responsible for proper cristae formation. Empirical data about this ubiquitous complex is limited to opisthokont models (e.g. yeast), impeding understanding of its core properties and adaptation potential to diverse cellular milieus. To close this knowledge gap, we characterized MICOS from the diverged excavate Trypanosoma brucei. These results allow the definition of fundamental MICOS properties, such as its role in maintaining both cristae and contacts between the inner and outer membranes plus its extrusion into the intermembrane space. Yet, surprising differences with opisthokonts indicate that MICOS can assume different forms. T. brucei MICOS contains a novel subunit involved in the import of intermembrane space proteins, including respiratory chain complex assembly factors. We propose an evidence-based hypothesis that T. brucei MICOS populates cristae membranes with proteins needed for respiration, supported by the complex's even distribution in cristae
  • SpeciesList: scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
  • ModificationList: TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
  • Instrument: LTQ Orbitrap Elite

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-04-25 08:26:06ID requested
12018-09-13 11:25:44announced

Publication List

  1. Dataset with its publication pending

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Mitochondria
  3. cristae
  4. intermembrane space
  5. MICOS
  6. protein import
  7. Trypanosoma brucei
  8. LC-MS/MS
  9. label-free
  10. TMT

Contact List

    Zbyněk Zdráhal
    • contact affiliation: CEITEC-MU, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic
    • contact email: zdrahal@sci.muni.cz
    • lab head:
    David Potesil
    • contact affiliation: Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic
    • contact email: david.potesil@ceitec.muni.cz
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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