PXD009584

PXD009584 is an original dataset announced via ProteomeXchange.

Title	Quantitative succinyl-proteome profiling of hickory (Carya cathayensis) during grafting process
Description	Hickory (Carya cathayensis) is an poplar nut-producing tree with high economic value. To overcome its long juvenile phase, grafting was applied to accelerate the transition from vegetative phase to reproductive phase. Lysine succinylation, a newly identified post-translational modification (PTM), is associated with various cellular processes. However, the regulatory mechanism underlying grafting process of hickory has not been studied at the level of PTM. Here, 259 succinylation sites in 202 proteins were identified, representing the first comprehensive lysine succinylome in hickory. The succinylation is biased to occur on the cytosolic proteins of hickory, indicating an effect of succinylation on the activities of enzymes associated with cellular metabolism. Moreover, four conserved succinylation motifs were identified in the succinylated peptides. Comparison of two grafting stages of hickory revealed that the differential expressed succinylated proteins were mainly involved in sugar metabolism, carbon fixation, amino acid metabolism and plant-pathogen interaction. In total, seven heat shock proteins (HSPs) with 11 succinylation sites were identified, and all these HSPs were up-regulated during the grafting process of hickory. Our data suggested that succinylated HSPs might play a role in the tolerance of grafted hickory plants. Our data are basic resources for the functional validation of succinylated proteins and a starting point for investigations into the molecular basis of lysine succinylation in the grafting process of hickory.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:04:42.071.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chenyu Lu
SpeciesList	scientific name: Carya cathayensis; NCBI TaxID: 139927;
ModificationList	N6-succinyl-L-lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-24 05:21:04	ID requested
1	2020-05-26 11:37:51	announced
⏵ 2	2024-10-22 05:04:44	announced	2024-10-22: Updated project metadata.

Yuan H, Chen J, Yang Y, Shen C, Xu D, Wang J, Yan D, He Y, Zheng B, Quantitative succinyl-proteome profiling of Chinese hickory (Carya cathayensis) during the grafting process. BMC Plant Biol, 19(1):467(2019) [pubmed]

10.1186/s12870-019-2072-8;

curator keyword: Biological

submitter keyword: succinylation, post-translational modification,hickory, grafting, heat shock protein

Bingsong Zheng
contact affiliation	Center for Cultivation of Subtropical Forest Resources (CCSFR), Zhejiang A & F University, Linan, Hangzhou, 311300, People's Republic of China.
contact email	bszheng@zafu.edu.cn
lab head
Chenyu Lu
contact affiliation	Zhejiang University
contact email	lcyyz@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD009584

PRIDE project URI

• PRIDE
  1. PXD009584
     1. Label: PRIDE project
     2. Name: Quantitative succinyl-proteome profiling of hickory (Carya cathayensis) during grafting process