PXD009582

PXD009582 is an original dataset announced via ProteomeXchange.

Title	Proteome allocation strategies under nutrient-limiting conditions in the cyanobacterium Synechocystis
Description	The response of the model cyanobacterium Synechocystis sp. PCC6803 towards light and carbon limitation was systematically probed. To this end, Synechocystis sp. PCC6803 was cultivated in a photo-bioreactor driven in turbidostat-mode. The turbidostat is a continuous cultivation that enabled cells to adapt to a constant environment, leading to a stable and 'optimal' proteome for the respective condition. The major dataset in this project consisted of 5 different 'concentrations' for light and CO2. Changes in the proteome were determined using using liquid chromatography/mass spectrometry and it was found that carbon and light limitation induced gradual but broad responses in gene expression. With decreasing substrate concentration (increasing limitation) a decrease in growth rate and a gradually more severe response in the proteome was visible.
HostingRepository	PRIDE
AnnounceDate	2018-09-12
AnnouncementXML	Submission_2018-10-15_02:55:33.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009582
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michael Jahn
SpeciesList	scientific name: Synechocystis sp. PCC 6803 substr. GT-S; NCBI TaxID: 1111707;
ModificationList	Oxidation; Carbamidomethyl
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-24 01:59:38	ID requested
1	2018-09-12 08:41:30	announced
⏵ 2	2018-10-15 02:55:34	announced	Updated publication reference for PubMed record(s): 30304686.

Jahn M, Vialas V, Karlsen J, Maddalo G, Edfors F, Forsstr, ö, m B, Uhl, é, n M, K, ä, ll L, Hudson EP, Growth of Cyanobacteria Is Constrained by the Abundance of Light and Carbon Assimilation Proteins. Cell Rep, 25(2):478-486.e8(2018) [pubmed]

curator keyword: Biological

submitter keyword: cyanobacteria, cellular economy, resource allocation, light limitation, carbon limitation, shotgun proteomics, data-dependent acquisition, openMS, MSGF+

Elton Paul Hudson
contact affiliation	Science for Life Laboratory, School of Biotechnology, KTH – Royal Institute of Technology, Stockholm, Sweden.
contact email	paul.hudson@scilifelab.se
lab head
Michael Jahn
contact affiliation	Science for Life Laboratory Stockholm
contact email	michael.jahn@scilifelab.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD009582

PRIDE project URI

• PRIDE
  1. PXD009582
     1. Label: PRIDE project
     2. Name: Proteome allocation strategies under nutrient-limiting conditions in the cyanobacterium Synechocystis