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PXD009575

PXD009575 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of MOSPD2, a novel scaffold for endoplasmic reticulum membrane contact sites
DescriptionMembrane contact sites are cellular structures that mediate inter-organelle exchange and communication. The endoplasmic reticulum (ER), which forms a network extending throughout the cytoplasm, possesses two known major tether proteins, named VAP-A and VAP-B, that allow its interaction with other organelles. VAP-A and VAP-B interact with proteins from other organelles that possess a small VAP-interacting motif, named FFAT (two phenylalanines in an acidic track), and consequently scaffold contact sites implicating the ER. In this study, by using an unbiased proteomic approach, we identified a novel ER tether named MOtile SPerm Domain-containing protein 2 (MOSPD2). We showed that MOSPD2 possesses a Motile Sperm Protein (MSP) domain which binds FFAT motifs and consequently allows membrane tethering in vitro. Accordingly, MOSPD2, which is an ER-anchored protein, interacts with several FFAT-containing proteins bound to diverse organelles,such as endosomes, mitochondria or Golgi. Consequently, the specific interaction between MOSPD2 and these organelle-bound proteins results in the formation of contact sites between the ER and these organelles. Thus, MOSPD2 is a novel tether for membrane contact sites between the ER and the other cellular organelles.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:44:46.698.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFabien Alpy
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-04-23 03:42:01ID requested
12018-06-05 08:54:55announced
22024-10-22 04:44:51announced2024-10-22: Updated project metadata.
Publication List
Di Mattia T, Wilhelm LP, Ikhlef S, Wendling C, Spehner D, Nomin, é Y, Giordano F, Mathelin C, Drin G, Tomasetto C, Alpy F, Identification of MOSPD2, a novel scaffold for endoplasmic reticulum membrane contact sites. EMBO Rep, 19(7):(2018) [pubmed]
10.15252/embr.201745453;
Keyword List
curator keyword: Biological
submitter keyword: endoplasmic reticulum,MOSPD2, FFAT motif
Contact List
Catherine Tomasetto
contact affiliation1 Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Functional Genomics and Cancer Department, Illkirch, France 2 Institut National de la Santé et de la Recherche Médicale (INSERM), U 1258, Illkirch, France 3 Centre National de la Recherche Scientifique (CNRS),UMR 7104, Illkirch, France 4 Université de Strasbourg, Illkirch, France
contact emailcat@igbmc.fr
lab head
Fabien Alpy
contact affiliationINSERM
contact emailfalpy@igbmc.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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