PXD009575

PXD009575 is an original dataset announced via ProteomeXchange.

Title	Identification of MOSPD2, a novel scaffold for endoplasmic reticulum membrane contact sites
Description	Membrane contact sites are cellular structures that mediate inter-organelle exchange and communication. The endoplasmic reticulum (ER), which forms a network extending throughout the cytoplasm, possesses two known major tether proteins, named VAP-A and VAP-B, that allow its interaction with other organelles. VAP-A and VAP-B interact with proteins from other organelles that possess a small VAP-interacting motif, named FFAT (two phenylalanines in an acidic track), and consequently scaffold contact sites implicating the ER. In this study, by using an unbiased proteomic approach, we identified a novel ER tether named MOtile SPerm Domain-containing protein 2 (MOSPD2). We showed that MOSPD2 possesses a Motile Sperm Protein (MSP) domain which binds FFAT motifs and consequently allows membrane tethering in vitro. Accordingly, MOSPD2, which is an ER-anchored protein, interacts with several FFAT-containing proteins bound to diverse organelles,such as endosomes, mitochondria or Golgi. Consequently, the specific interaction between MOSPD2 and these organelle-bound proteins results in the formation of contact sites between the ER and these organelles. Thus, MOSPD2 is a novel tether for membrane contact sites between the ER and the other cellular organelles.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:46.698.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabien Alpy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-23 03:42:01	ID requested
1	2018-06-05 08:54:55	announced
⏵ 2	2024-10-22 04:44:51	announced	2024-10-22: Updated project metadata.

Di Mattia T, Wilhelm LP, Ikhlef S, Wendling C, Spehner D, Nomin, é Y, Giordano F, Mathelin C, Drin G, Tomasetto C, Alpy F, Identification of MOSPD2, a novel scaffold for endoplasmic reticulum membrane contact sites. EMBO Rep, 19(7):(2018) [pubmed]

10.15252/embr.201745453;

curator keyword: Biological

submitter keyword: endoplasmic reticulum,MOSPD2, FFAT motif

Catherine Tomasetto
contact affiliation	1 Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Functional Genomics and Cancer Department, Illkirch, France 2 Institut National de la Santé et de la Recherche Médicale (INSERM), U 1258, Illkirch, France 3 Centre National de la Recherche Scientifique (CNRS),UMR 7104, Illkirch, France 4 Université de Strasbourg, Illkirch, France
contact email	cat@igbmc.fr
lab head
Fabien Alpy
contact affiliation	INSERM
contact email	falpy@igbmc.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD009575

PRIDE project URI

• PRIDE
  1. PXD009575
     1. Label: PRIDE project
     2. Name: Identification of MOSPD2, a novel scaffold for endoplasmic reticulum membrane contact sites