PXD009563

PXD009563 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis thaliana responds to colonisation of Piriformospora indica by secretion of symbiosis specific proteins
Description	Plants interact with a wide variety of fungi in a mutualistic, parasitic or neutral way. The associations formed depend on the exchange of nutrients and signalling molecules between the partners. The latter include a diverse set of protein classes, of which some have been demonstrated to be functionally implicated in defence, nutrient uptake and establishing a symbiotic relationship. Here, we have analysed the secretomes of the mutualistic, root-endophytic fungus Piriformospora indica and Arabidopsis thaliana when cultivated alone or in a co-culture. More than one hundred proteins were identified as differentially secreted, including proteins associated with growth, development, abiotic and biotic stress response and mucilage. One protein found in the co-culture is PLAT1 (Polycystin, Lipoxygenase, Alpha-toxin and Triacylglycerol lipases). PLAT1 has not been associated with plant-fungal-interaction before and is known to play a role in abiotic stress responses. It co-localizes with Brassicaceae-specific endoplasmic reticulum bodies (ER bodies), which are involved in the formation of the defence compound scopolin. We observed degraded ER bodies in P. indica infected Arabidopsis roots and plat1 mutants were stronger colonised than wild tyype (WT) plants. Furthermore, PLAT1 RNA (ribonucleic acid) and scopolin levels were detected to be regulated by the fungus in a stage-specific manner.
HostingRepository	PRIDE
AnnounceDate	2019-01-07
AnnouncementXML	Submission_2019-01-07_01:57:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Krüger
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Piriformospora sp.; NCBI TaxID: 2011957;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-23 00:46:42	ID requested
⏵ 1	2019-01-07 01:57:40	announced

Th, ü, rich J, Meichsner D, Furch ACU, Pfalz J, Kr, ü, ger T, Kniemeyer O, Brakhage A, Oelm, ü, ller R, Arabidopsis thaliana responds to colonisation of Piriformospora indica by secretion of symbiosis-specific proteins. PLoS One, 13(12):e0209658(2018) [pubmed]

curator keyword: Biological

submitter keyword: Piriformospora indica, secretome, ER body, scopolin, PLAT

Axel A. Brakhage
contact affiliation	Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact email	axel.brakhage@leibniz-hki.de
lab head
Thomas Krüger
contact affiliation	Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact email	thomas.krueger@leibniz-hki.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009563
     1. Label: PRIDE project
     2. Name: Arabidopsis thaliana responds to colonisation of Piriformospora indica by secretion of symbiosis specific proteins