PXD009561

PXD009561 is an original dataset announced via ProteomeXchange.

Title	Extended ZIKV-NS4B interactome (AP-MS/MS analysis of ZIKV interacting proteins)
Description	To identify the cellular interactors of ZIKV-NS4B-interacting proteins and their differential composition upon ZIKV infection, individual cDNAs expressing FLAG-tagged variants of the following ZIKV-NS4B-interacting proteins were generated (GeneBank and UniProt Accession numbers are given in parenthesis): CEND1 (BC034732.1; Q8N111), CHP1 (BC031293; Q99653), CLN6 (BC010849.1; Q9NWW5), TMEM41b (BC035034.1; Q5BJD5), RBFOX2 (NM_001082579.1; O43251-6), BSG (BC009040.2;P35613). Lentiviruses expressing each of these proteins were used to transduce SK-N-BE2 cells, which were either mock-infected or infected with ZIKV (H/PF/2013, MOI=3), and 72 hours later subjected to AP-MS/MS to identify the respective cellular interactors.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:39.484.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pietro Scaturro
SpeciesList	scientific name: Zika virus ZIKV/H. sapiens/FrenchPolynesia/10087PF/2013; NCBI TaxID: 2043570; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-20 07:35:01	ID requested
1	2018-09-03 00:43:52	announced
2	2018-09-05 04:30:42	announced	Updated publication reference for PubMed record(s): 30177828.
⏵ 3	2024-10-22 04:44:47	announced	2024-10-22: Updated project metadata.

10.1038/s41586-018-0484-5;

Scaturro P, Stukalov A, Haas DA, Cortese M, Draganova K, P, ł, aszczyca A, Bartenschlager R, G, ö, tz M, Pichlmair A, An orthogonal proteomic survey uncovers novel Zika virus host factors. Nature, 561(7722):253-257(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: CEND1, TMEM41b, RBFOX2, CHP1, ZIKV, AP-MS/MS,CLN6, LC-MS/MS

Andreas Pichlmair
contact affiliation	Immunopathology of Virus Infections Institute of Virology Technical University of Munich Munich, Germany
contact email	andreas.pichlmair@tum.de
lab head
Pietro Scaturro
contact affiliation	Systems Arbovirology, Leibniz Institute of Virology
contact email	pietro.scaturro@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD009561

PRIDE project URI

• PRIDE
  1. PXD009561
     1. Label: PRIDE project
     2. Name: Extended ZIKV-NS4B interactome (AP-MS/MS analysis of ZIKV interacting proteins)