PXD009549

PXD009549 is an original dataset announced via ProteomeXchange.

Title	Comprehensive biochemical characterization of Mycobacterium tuberculosis clonal strains after isoniazid resistance
Description	This project describes the isoniazid (INH) resistance acquisition event in Mycobacterium tuberculosis (Mtb) from the proteomics perspective. In this way, an exploration of the protein differences, comparing clonal INH susceptible (INHs) and INHr pairs of Mtb were evaluated. One clonal clinical and one clonal laboratory-derived Mtb pair with different susceptibility profiles to INH were studied. The laboratory INHr strain had one katG mutation (V1A), while the clinical INHr strain had two (V1A and E3V). Large-scale bacterial cultures were grown in triplicate to obtain secreted proteins as well as proteins from cell fractions. The resulting peptide solutions from all fractions were analyzed using liquid-chromatography coupled with tandem mass spectrometry (LC-MS/MS). LC-MS/MS spectra were compared against an Mtb database to determine the protein abundance. Protein abundance differences were tested by Student’s t test. Looking at the same cellular fractions, there were 25 commonly altered Mtb proteins after acquiring INH resistance. These proteins were involved in ATP synthase machinery, lipid metabolism, regulatory events, virulence, detoxification and adaptation processes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:41.781.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009549
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Carolina Mehaffy
SpeciesList	scientific name: Mycobacterium tuberculosis; NCBI TaxID: 1773;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-20 01:32:06	ID requested
1	2018-06-25 06:13:28	announced
⏵ 2	2024-10-22 04:44:45	announced	2024-10-22: Updated project metadata.

10.1074/mcp.ra118.000821;

Nieto R LM, Mehaffy C, Islam MN, Fitzgerald B, Belisle J, Prenni J, Dobos K, Can the Analysis of Clonal Strains Reveal Novel Targetable Pathways? Mol Cell Proteomics, 17(9):1685-1701(2018) [pubmed]

10.6019/PXD009549;

curator keyword: Biological, Biomedical

submitter keyword: Mycobacterium tuberculosis, isoniazid resistance

Karen M Dobos
contact affiliation	Director, Research Integrity and Compliance Review Office (http://ricro.colostate.edu/) Associate Professor, Mycobacteria Research Laboratories Dept of Microbiology, Immunology,& Pathology (http://www.cvmbs.colostate.edu/DirectorySearch/Search/MemberProfile/CVMBS/3607) Colorado State University Ft. Collins, CO 80523, USA
contact email	karen.dobos@colostate.edu
lab head
Carolina Mehaffy
contact affiliation	Colorado State University
contact email	carolina.mehaffy@colostate.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD009549

PRIDE project URI

• PRIDE
  1. PXD009549
     1. Label: PRIDE project
     2. Name: Comprehensive biochemical characterization of Mycobacterium tuberculosis clonal strains after isoniazid resistance