PXD009539

PXD009539 is an original dataset announced via ProteomeXchange.

Title	Destabilization of the MiniChromosome Maintenance (MCM) complex modulates the cellular response to DNA double strand breaks induced by Etoposide.
Description	DNA replication during S phase involves thousands of replication forks that must be coordinated to ensure that every DNA section is only replicated once. The minichromosome maintenance proteins, MCM2 to MCM7, form a heteromeric DNA helicase required for both initiation of DNA replication and elongation of DNA replication. Although only two DNA helicase activities are required to establish a bidirectional replication fork from each origin of replication, a large excess of MCM complexes is loaded and distributed along the chromatin. The function of the additional MCM complexes is not well understood, as most of them are displaced from the DNA during S-phase, apparently without playing an active role in DNA replication. DNA damage response (DDR) kinases activated by stalled forks prevent the replication machinery from being activated, indicating a tight relationship between DDR and DNA replication. To investigate the role of MCM proteins in the cellular response to DNA damage, we used shRNA targeting MCM2 or MCM3 to determine the impact of the reduction of the MCM complex. The alteration of MCM proteins induced a change in the activation of important factors of the DDR in response to Etoposide treatment. The phosphorylation of γ-H2AX, CHK1 and CHK2 is affected following DNA damage induced by treatment with Etoposide without affecting cell viability. Using assays measuring homologous recombination (HR) and non-homologous end-joining (NHEJ), we have identified a decrease of HR, but not NHEJ, associated with a decrease of the MCM complex demonstrating a role for the MCM complex in homologous recombination.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:26.098.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francois-Michel Boisvert
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; 6x(13)C labeled residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-19 01:41:40	ID requested
1	2019-11-12 10:54:05	announced
⏵ 2	2024-10-22 04:44:34	announced	2024-10-22: Updated project metadata.

Drissi R, Chauvin A, McKenna A, L, é, vesque D, Blais-Brochu S, Jean D, Boisvert FM, Destabilization of the MiniChromosome Maintenance (MCM) complex modulates the cellular response to DNA double strand breaks. Cell Cycle, 17(23):2593-2609(2018) [pubmed]

10.1080/15384101.2018.1553336;

curator keyword: Biological

submitter keyword: DNA repair,MCM protein, Etoposide, DNA damage, Homologous Recombination

Francois-Michel Boisvert
contact affiliation	Department of Anatomy and Cell Biology, Université de Sherbrooke
contact email	fm.boisvert@usherbrooke.ca
lab head
Francois-Michel Boisvert
contact affiliation	Université de Sherbrooke
contact email	fm.boisvert@usherbrooke.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD009539

PRIDE project URI

• PRIDE
  1. PXD009539
     1. Label: PRIDE project
     2. Name: Destabilization of the MiniChromosome Maintenance (MCM) complex modulates the cellular response to DNA double strand breaks induced by Etoposide.