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PXD009529

PXD009529 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGlobal profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome
DescriptionThe RNA exosome is a multi-subunit complex essential for processing and degradation of many classes of RNA. Although many of the functions of the RNA exosome are well established, how the activity of this complex is regulated remains poorly understood. Here we report a comprehensive proteomic analysis of the RNA exosome complex from the fission yeast Schizosaccharomyces pombe and identified 39 post-translational modifications (PTMs), including phosphorylation, methylation, and acetylation. Interestingly, most of the modifications were identified in Dis3 and Rrp6, the catalytic subunits of the RNA exosome, as well as in the exosome-associated RNA helicase, Mtr4. Functional characterization of residues found to be modified in distinct exosome subunits revealed specific substitutions that affected cell growth and RNA exosome functions. Notably, phosphomimetic substitutions of phosphorylation sites Ser-809 and Tyr-814 in S. pombe Dis3 resulted in striking loss-of-function phenotype in vivo. Biochemical analysis of the Ser-809 and Tyr-814 phosphomimetic versions of Dis3 revealed proper assembly in vivo, but a marked decrease in degradation capacity in vitro. Given that Ser-809 and Tyr-814 are positioned in the vicinity of the catalytic centre of Dis3, our data suggest that site-specific phosphorylation of the RNA exosome represents a mechanism to control its activity in vivo.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:44:33.002.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCaroline Telekawa
SpeciesList scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690;
ModificationListmonomethylated residue; phosphorylated residue; acetylated residue; dimethylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-04-18 02:08:56ID requested
12019-01-21 05:11:51announced
22024-10-22 04:44:39announced2024-10-22: Updated project metadata.
Publication List
Telekawa C, Boisvert FM, Bachand F, Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome. Nucleic Acids Res, 46(21):11169-11183(2018) [pubmed]
10.1093/nar/gky915;
Keyword List
curator keyword: Biological
submitter keyword: fission yeast,post-translational modification, RNA exosome, gene expression regulation
Contact List
François Bachand
contact affiliationRNA Group, Department of Biochemistry, Université de Sherbrooke, Sherbrooke, Qc, Canada
contact emailfrancois.bachand2@usherbrooke.ca
lab head
Caroline Telekawa
contact affiliationUniversité de Sherbrooke
contact emailcaroline.telekawa@usherbrooke.ca
dataset submitter
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