PXD009529

PXD009529 is an original dataset announced via ProteomeXchange.

Title	Global profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome
Description	The RNA exosome is a multi-subunit complex essential for processing and degradation of many classes of RNA. Although many of the functions of the RNA exosome are well established, how the activity of this complex is regulated remains poorly understood. Here we report a comprehensive proteomic analysis of the RNA exosome complex from the fission yeast Schizosaccharomyces pombe and identified 39 post-translational modifications (PTMs), including phosphorylation, methylation, and acetylation. Interestingly, most of the modifications were identified in Dis3 and Rrp6, the catalytic subunits of the RNA exosome, as well as in the exosome-associated RNA helicase, Mtr4. Functional characterization of residues found to be modified in distinct exosome subunits revealed specific substitutions that affected cell growth and RNA exosome functions. Notably, phosphomimetic substitutions of phosphorylation sites Ser-809 and Tyr-814 in S. pombe Dis3 resulted in striking loss-of-function phenotype in vivo. Biochemical analysis of the Ser-809 and Tyr-814 phosphomimetic versions of Dis3 revealed proper assembly in vivo, but a marked decrease in degradation capacity in vitro. Given that Ser-809 and Tyr-814 are positioned in the vicinity of the catalytic centre of Dis3, our data suggest that site-specific phosphorylation of the RNA exosome represents a mechanism to control its activity in vivo.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:33.002.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Caroline Telekawa
SpeciesList	scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690;
ModificationList	monomethylated residue; phosphorylated residue; acetylated residue; dimethylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-18 02:08:56	ID requested
1	2019-01-21 05:11:51	announced
⏵ 2	2024-10-22 04:44:39	announced	2024-10-22: Updated project metadata.

Telekawa C, Boisvert FM, Bachand F, Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome. Nucleic Acids Res, 46(21):11169-11183(2018) [pubmed]

10.1093/nar/gky915;

curator keyword: Biological

submitter keyword: fission yeast,post-translational modification, RNA exosome, gene expression regulation

François Bachand
contact affiliation	RNA Group, Department of Biochemistry, Université de Sherbrooke, Sherbrooke, Qc, Canada
contact email	francois.bachand2@usherbrooke.ca
lab head
Caroline Telekawa
contact affiliation	Université de Sherbrooke
contact email	caroline.telekawa@usherbrooke.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD009529

PRIDE project URI

• PRIDE
  1. PXD009529
     1. Label: PRIDE project
     2. Name: Global profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome