PXD009522

PXD009522 is an original dataset announced via ProteomeXchange.

Title	The circadian E3 ligase complex SCFFBXL3+CRY targets TLK2
Description	We recently demonstrated that the circadian clock component CRY2 is an essential cofactor in the SCFFBXL3-mediated ubiquitination of c-MYC. Because our demonstration that CRY2 recruits phosphorylated substrates to SCFFBXL3 was unexpected, we investigated the scope of this role by searching for additional substrates of FBXL3 that require CRY1 or CRY2 as cofactors. Here, we describe an affinity purification mass spectrometry (APMS) screen through which we identified more than one hundred potential substrates of SCFFBXL3+CRY1/2, including the cell cycle regulated Tousled-like kinase, TLK2. Both CRY1 and CRY2 recruit TLK2 to SCFFBXL3, and TLK2 kinase activity is required for this interaction. Overexpression or genetic deletion of CRY1 and/or CRY2 decreases or enhances TLK2 protein abundance, respectively. These findings reinforce the idea that CRYs function as co-factors for SCFFBXL3, provide a resource of potential substrates, and establish a molecular connection between the circadian and cell cycle oscillators via CRY-modulated turnover of TLK2.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_10:58:35.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009522
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	James Moresco
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-17 01:30:59	ID requested
⏵ 1	2019-11-12 10:58:37	announced

Correia SP, Chan AB, Vaughan M, Zolboot N, Perea V, Huber AL, Kriebs A, Moresco JJ, Yates JR, Lamia KA, targets TLK2. Sci Rep, 9(1):198(2019) [pubmed]

curator keyword: Biological

submitter keyword: Circadian Clock

Katja A. Lamia
contact affiliation	Department of Molecular Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
contact email	klamia@scripps.edu
lab head
James Moresco
contact affiliation	The Salk Institute for Biological Studies
contact email	jmoresco@salk.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD009522

PRIDE project URI

• PRIDE
  1. PXD009522
     1. Label: PRIDE project
     2. Name: The circadian E3 ligase complex SCFFBXL3+CRY targets TLK2