PXD009518

PXD009518 is an original dataset announced via ProteomeXchange.

Title	SILAC APMS analysis of RPAP3 C-term interactome
Description	To get insights into the function of RPAP3, we characterized its partners by performing a proteomic analysis in human cells. We fused the Cterm part of RPAP3 domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP-RPAP3-Cter and control cells with isotopically labelled amino-acids (SILAC), whole cell extracts were immuno-precipitated (IP) with anti-GFP antibodies and immunoprecipitates were subjected to quantitative mass-spectrometry analysis. Similar experiments were then performed on two mutated form of RPAP3 that can no longer with the two essential AAA+ ATPases RUVBL1/RUVBL2, important partners of Wild type RPAP3 Cterm. 3 batchs of SILAC analysis K0R0 control Hela H9 vs K4R6 x-FLAG- GFP- RPAP3 Cterm Wild type K0R0 control Hela H9 vs K4R6 x-FLAG- GFP- RPAP3 Cterm mutant 1 R623A-M626A K0R0 control Hela H9 vs K4R6 x-FLAG- GFP- RPAP3 Cterm mutant 2 F630A-S632A
HostingRepository	PRIDE
AnnounceDate	2018-06-10
AnnouncementXML	Submission_2018-06-10_08:26:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Franck Vandermoere
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	isotope labeled residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-16 05:37:26	ID requested
⏵ 1	2018-06-10 08:26:37	announced

Maurizy C, Quinternet M, Abel Y, Verheggen C, Santo PE, Bourguet M, C F Paiva A, Bragantini B, Chagot ME, Robert MC, Abeza C, Fabre P, Fort P, Vandermoere F, M F Sousa P, Rain JC, Charpentier B, Cianf, é, rani S, Bandeiras TM, Pradet-Balade B, Manival X, Bertrand E, The RPAP3-Cterminal domain identifies R2TP-like quaternary chaperones. Nat Commun, 9(1):2093(2018) [pubmed]

curator keyword: Biological

submitter keyword: Human, Hela cells, SILAC, APMS, LTQ-Orbitrap

Edouard Bertrand
contact affiliation	IGMM, CNRS, Université de Montpellier, Montpellier, France
contact email	edouard.bertrand@igmm.cnrs.fr
lab head
Franck Vandermoere
contact affiliation	IGF (Montpellier, France), CNRS, INSERM, University of Montpellier
contact email	franck.vandermoere@igf.cnrs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD009518

PRIDE project URI

• PRIDE
  1. PXD009518
     1. Label: PRIDE project
     2. Name: SILAC APMS analysis of RPAP3 C-term interactome