PXD009516

PXD009516 is an original dataset announced via ProteomeXchange.

Title	Interactome of Ca2+-bound secretagogin in mouse embryonic (E13.5) pancreas
Description	By using His6-tagged recombinant human secretagogin in the presence of Ca2+ (100 µM), we isolated its putative interacting partners from mouse embryonic (E13.5) pancreata and identified their amino acid sequences by mass spectrometry. Next to previously described interacting partners (participating in vesicle-mediated transport, cytoskeletal organization and members of chaperonin-containing complex), we found a substantial group of proteins involved in proteasomal catabolic process including 15 different subunits of the 26S proteasome. Our data support that secretagogin protects Pdx1 from proteasomal degradation to control a transcriptional program required for β cell specification.
HostingRepository	PRIDE
AnnounceDate	2018-06-13
AnnouncementXML	Submission_2018-06-28_00:58:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Edit Szodorai
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-16 04:09:04	ID requested
1	2018-06-13 03:47:32	announced
⏵ 2	2018-06-28 00:58:09	announced	Updated publication reference for PubMed record(s): 29910119.

Malenczyk K, Szodorai E, Schnell R, Lubec G, Szab, ó G, H, ö, kfelt T, Harkany T, cell specification. Mol Metab, 14():108-120(2018) [pubmed]

curator keyword: Biological

submitter keyword: His6-tagged recombinant secretagogin, pull down, mouse embryonic (E13.5) pancreas, protein-protein interactions

Univ. Prof. Dr. Tibor Harkany
contact affiliation	1 Department of Molecular Neurosciences, Center for Brain Research, Medical University of Vienna, Spitalgasse 4, A-1090 Vienna, Austria; 2 Department of Neuroscience, Karolinska Institutet, Retzius väg 8, SE-17177 Stockholm, Sweden
contact email	tibor.harkany@meduniwien.ac.at
lab head
Edit Szodorai
contact affiliation	Dept. of Molecular Neurosciences, Center for Brain Research, Medical University of Vienna, Vienna, Austria
contact email	edit.szodorai@meduniwien.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD009516

PRIDE project URI

• PRIDE
  1. PXD009516
     1. Label: PRIDE project
     2. Name: Interactome of Ca2+-bound secretagogin in mouse embryonic (E13.5) pancreas