PXD009515

PXD009515 is an original dataset announced via ProteomeXchange.

Title	MT-MAMS: protein methyltransferase motif analysis by mass spectrometry
Description	Protein methyltransferases can recognise their substrates through linear sequence motifs and determination of these motifs is critical to understand methyltransferase mechanism, function and drug targeting. Here we describe MT-MAMS (methyltransferase motif analysis by mass spectrometry), a quantitative approach to characterise methyltransferase substrate recognition motifs and enzyme processivity. We validated MT-MAMS by application to lysine methyltransferase G9a, then determined the recognition motifs of Efm1 and major arginine methyltransferases Hmt1 and PRMT1.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:45.688.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joshua Hamey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monomethylated residue; trimethylated residue; dimethylated residue
Instrument	Orbitrap Fusion Lumos; LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-16 03:57:12	ID requested
1	2018-09-03 02:32:42	announced
⏵ 2	2024-10-22 04:44:53	announced	2024-10-22: Updated project metadata.

Hamey JJ, Separovich RJ, Wilkins MR, MT-MAMS: Protein Methyltransferase Motif Analysis by Mass Spectrometry. J Proteome Res, 17(10):3485-3491(2018) [pubmed]

10.1021/acs.jproteome.8b00396;

curator keyword: Technical, Biological

submitter keyword: motif,Methylation, methyltransferase

Marc R Wilkins
contact affiliation	School of Biotechnology and Biomolecular Sciences, University of New South Wales, New South Wales, 2052, Australia
contact email	m.wilkins@unsw.edu.au
lab head
Joshua Hamey
contact affiliation	University of New South Wales
contact email	j.hamey@unsw.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD009515

PRIDE project URI

• PRIDE
  1. PXD009515
     1. Label: PRIDE project
     2. Name: MT-MAMS: protein methyltransferase motif analysis by mass spectrometry