PXD009509

PXD009509 is an original dataset announced via ProteomeXchange.

Title	Remodelling of the acetylproteome by SIRT3 manipulation fails to affect insulin secretion or beta-cell metabolism in the absence of over nutrition
Description	From Peterson et. al. 2018: SIRT3 is an NAD+-dependent mitochondrial protein deacetylase purported to influence metabolism through post-translational modification of metabolic enzymes. Fuel-stimulated insulin secretion, which involves mitochondrial metabolism, could be susceptible to SIRT3-mediated effects. We used CRISPR/Cas9 technology to manipulate SIRT3 expression in b-cells, resulting in wide-spread, SIRT3dependent changes in acetylation of key metabolic enzymes, but with no appreciable changes in glucose- or pyruvate-stimulated insulin secretion, or in metabolomic profile during glucose stimulation. Moreover, these broad changes in the SIRT3-targeted acetylproteome did not affect responses to nutritional or ER stress conditions. We also studied mice with global SIRT3 knockout fed either standard chow (STD) or high- fat/high-sucrose (HFHS) diets. Only when chronically fed a HFHS diet do SIRT3 KO animals exhibit a modest reduction in insulin secretion. We conclude that broad changes in mitochondrial protein acetylation in response to manipulation of SIRT3 are not sufficient to cause changes in islet function or metabolism.
HostingRepository	PRIDE
AnnounceDate	2018-06-23
AnnouncementXML	Submission_2018-07-11_08:04:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paul Grimsrud
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-16 02:05:29	ID requested
1	2018-06-21 07:51:26	announced
2	2018-06-23 00:30:15	announced	Updated project metadata.
⏵ 3	2018-07-11 08:04:16	announced	Updated project metadata.

Peterson BS, Campbell JE, Ilkayeva O, Grimsrud PA, Hirschey MD, Newgard CB, Cell Metabolism in the Absence of Overnutrition. Cell Rep, 24(1):209-223.e6(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: SIRT3, protein acetylation, beta cells, pancreas

Christopher B. Newgard
contact affiliation	Director, Duke Molecular Physiology Institute, Professor Departments of Medicine (Division of Endocrinology, Metabolism, and Nutrition) and Pharmacology & Cancer Biology Duke University Medical Center
contact email	chris.newgard@duke.edu
lab head
Paul Grimsrud
contact affiliation	Duke Molecular Physiology Institute
contact email	paul.grimsrud@duke.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD009509

PRIDE project URI

• PRIDE
  1. PXD009509
     1. Label: PRIDE project
     2. Name: Remodelling of the acetylproteome by SIRT3 manipulation fails to affect insulin secretion or beta-cell metabolism in the absence of over nutrition