PXD009500

PXD009500 is an original dataset announced via ProteomeXchange.

Title	Bi-directional cell-pericellular matrix interactions direct stem cell fate
Description	Modifiable hydrogels have revealed tremendous insight into how physical characteristics of cells’ 3D environment drive stem cell lineage specification. However, in native tissues, cells do not passively receive signals from their niche. Instead they actively probe and modify their pericellular space to suit their needs, yet the dynamics of cells’ reciprocal interactions with their pericellular matrix when encapsulated within hydrogels remains relatively unexplored. Here, we show that human bone marrow stromal cells (hMSC) encapsulated within hyaluronic acid-based hydrogels modify their pericellular environment through degradation and/or protein secretion, imparting them with similar pericellular stiffnesses, regardless of initial hydrogel properties. These cell-secreted pericellular matrices play a role in regulating hMSC fate, with secretion of a stiff proteinaceous pericellular matrix associated with adipogenesis, and degradation with osteogenesis. Our observations suggest that hMSC participate in a bi-directional interplay between the properties of their 3D milieu and their own secreted pericellular matrix, and that this combination of interactions drives fate.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:36.685.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Faull
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-13 07:24:47	ID requested
1	2018-11-26 02:45:23	announced
⏵ 2	2024-10-22 04:44:44	announced	2024-10-22: Updated project metadata.

10.1038/s41467-018-06183-4;

Ferreira SA, Motwani MS, Faull PA, Seymour AJ, Yu TTL, Enayati M, Taheem DK, Salzlechner C, Haghighi T, Kania EM, Oommen OP, Ahmed T, Loaiza S, Parzych K, Dazzi F, Varghese OP, Festy F, Grigoriadis AE, Auner HW, Snijders AP, Bozec L, Gentleman E, Bi-directional cell-pericellular matrix interactions direct stem cell fate. Nat Commun, 9(1):4049(2018) [pubmed]

curator keyword: Biological

submitter keyword: stem cells, matrix proteins, SILAC, Lumos, orbitrap, interactions, fate

Bram Snijders
contact affiliation	Protein Analysis and Proteomics Platform The Francis Crick Institute London NW1 1AT UK
contact email	bram.snijders@crick.ac.uk
lab head
Peter Faull
contact affiliation	The Francis Crick Institute
contact email	peter.faull@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD009500

PRIDE project URI

• PRIDE
  1. PXD009500
     1. Label: PRIDE project
     2. Name: Bi-directional cell-pericellular matrix interactions direct stem cell fate