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PXD009494 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleYeast genetic codes
DescriptionWhile the “universal” genetic code is now known not to be universal, and stop codons can have multiple meanings, one regularity remains, namely that for a given sense codon there is a unique translation. Examining CUG usage in yeasts that have transferred CUG away from leucine, we here report the first example of dual coding: Ascoidea asiatica stochastically encodes CUG as both serine and leucine. This is deleterious as evidenced by CUG codons being rare, never at conserved serine/leucine residues and predominantly in lowly expressed genes. Related yeasts solve the problem by loss-of-function of one of the two tRNAs. This dual-coding is consistent with the tRNA-loss driven codon reassignment hypothesis and provides a unique example of a proteome that cannot be deterministically predicted.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMartin Kollmar
SpeciesList scientific name: Babjeviella inositovora NRRL Y-12698; NCBI TaxID: 984486; scientific name: Nakazawaea peltata; NCBI TaxID: 1538182; scientific name: Ascoidea rubescens DSM 1968; NCBI TaxID: 1344418; scientific name: Clavispora lusitaniae ATCC 42720; NCBI TaxID: 306902; scientific name: Saccharomycopsis malanga; NCBI TaxID: 44092; scientific name: Ascoidea asiatica; NCBI TaxID: 1301101; scientific name: Saccharomycopsis fibuligera; NCBI TaxID: 4944;
ModificationListN-acetylated residue; monohydroxylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-04-13 02:19:21ID requested
12018-06-13 07:40:28announced
22018-06-20 00:20:17announcedUpdated publication reference for PubMed record(s): 29910077.
Publication List
Mühlhausen S, Schmitt HD, Pan KT, Plessmann U, Urlaub H, Hurst LD, Kollmar M, Endogenous Stochastic Decoding of the CUG Codon by Competing Ser- and Leu-tRNAs in Ascoidea asiatica. Curr Biol, 28(13):2046-2057.e5(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Ascoidea, Saccharomycopsis, Nakazawaea, Babjeviella, Clavispora
Contact List
Martin Kollmar
contact affiliationGroup Systems Biology of Motor Proteins, Department of NMR-based Structural Biology, Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany
contact emailmako@nmr.mpibpc.mpg.de
lab head
Martin Kollmar
contact affiliationMPI for Biophysical Chemistry
contact emailmako@nmr.mpibpc.mpg.de
dataset submitter
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