PXD009488 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Protein profiling of stress-activated breast cancer cell models |
Description | This study provides insights into the efficacy of beta-blockers as breast cancer therapeutics.Cell line models of basal-type and estrogen receptor-positive breast cancer were profiled for basal levels of adrenoceptor gene/protein expression, and ADRβ2-mediated cell behaviour including migration, invasion, adhesion, and proliferation in response to adrenoceptor agonist/antagonist treatment. Protein profiling and histology identified response biomarkers and drug targets. Protein profiling revealed the upregulation of the pro-metastatic gene LYPD3 in norepinephrine treated MDA MB 468 cells. Histology confirmed selective LYPD3 expression in clinical primary and metastatic breast tumours. These findings demonstrate that basal-type cancer models show a more aggressive ADRβ2-activated phenotype in the resting and stimulated state, which is attenuated by ADRβ2 inhibition, and explain some of the previous studies that have cast doubt on the value of beta-blocker therapy in breast cancer. These findings suggest that propranolol should be clinically evaluated in patients with basal-type tumours expressing high levels of ADRβ2 and LYPD3. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:04:43.721.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Amanda Miles |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-04-13 01:15:54 | ID requested | |
1 | 2020-05-26 11:50:34 | announced | |
⏵ 2 | 2024-10-22 05:04:52 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.3390/biology9020039; |
Gruet M, Cotton D, Coveney C, Boocock DJ, Wagner S, Komorowski L, Rees RC, Pockley AG, Garner AC, Wallis JD, Miles AK, Powe DG, 2-Adrenergic Signalling Promotes Cell Migration by Upregulating Expression of the Metastasis-Associated Molecule LYPD3. Biology (Basel), 9(2):(2020) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: breast cancer, LC-MSMS, SWATH,human |
Contact List
David J Boocock |
contact affiliation | Nottingham Trent University, John van Geest Cancer Research Centre. |
contact email | david.boocock@ntu.ac.uk |
lab head | |
Amanda Miles |
contact affiliation | Nottingham Trent University |
contact email | amanda.miles@ntu.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009488
- Label: PRIDE project
- Name: Protein profiling of stress-activated breast cancer cell models