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PXD009488

PXD009488 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein profiling of stress-activated breast cancer cell models
DescriptionThis study provides insights into the efficacy of beta-blockers as breast cancer therapeutics.Cell line models of basal-type and estrogen receptor-positive breast cancer were profiled for basal levels of adrenoceptor gene/protein expression, and ADRβ2-mediated cell behaviour including migration, invasion, adhesion, and proliferation in response to adrenoceptor agonist/antagonist treatment. Protein profiling and histology identified response biomarkers and drug targets. Protein profiling revealed the upregulation of the pro-metastatic gene LYPD3 in norepinephrine treated MDA MB 468 cells. Histology confirmed selective LYPD3 expression in clinical primary and metastatic breast tumours. These findings demonstrate that basal-type cancer models show a more aggressive ADRβ2-activated phenotype in the resting and stimulated state, which is attenuated by ADRβ2 inhibition, and explain some of the previous studies that have cast doubt on the value of beta-blocker therapy in breast cancer. These findings suggest that propranolol should be clinically evaluated in patients with basal-type tumours expressing high levels of ADRβ2 and LYPD3.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:04:43.721.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAmanda Miles
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-04-13 01:15:54ID requested
12020-05-26 11:50:34announced
22024-10-22 05:04:52announced2024-10-22: Updated project metadata.
Publication List
10.3390/biology9020039;
Gruet M, Cotton D, Coveney C, Boocock DJ, Wagner S, Komorowski L, Rees RC, Pockley AG, Garner AC, Wallis JD, Miles AK, Powe DG, 2-Adrenergic Signalling Promotes Cell Migration by Upregulating Expression of the Metastasis-Associated Molecule LYPD3. Biology (Basel), 9(2):(2020) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: breast cancer, LC-MSMS, SWATH,human
Contact List
David J Boocock
contact affiliationNottingham Trent University, John van Geest Cancer Research Centre.
contact emaildavid.boocock@ntu.ac.uk
lab head
Amanda Miles
contact affiliationNottingham Trent University
contact emailamanda.miles@ntu.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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