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PXD009487

PXD009487 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGlobal changes in mRNA abundance drive differential shuttling of RNA binding proteins, linking cytoplasmic RNA degradation to transcription
DescriptionAlterations in global mRNA decay can broadly impact multiple upstream and downstream stages of gene expression. For example, accelerated cytoplasmic mRNA degradation can trigger a reduction in mammalian RNA polymerase II (RNAPII) transcription, although signals that connect these seemingly distal processes remain largely unknown. Here, we used tandem mass tag labeling with mass spectrometry to chart how changes in Xrn1-dependent mRNA degradation impact nuclear-cytoplasmic protein distribution in human cells. Notably, accelerating mRNA decay through expression of a gammaherpesviral endonuclease known to coordinate with Xrn1 drove nuclear relocalization of many RNA binding proteins. Particularly enriched in the relocalized subset were factors linked to the poly(A) tail. Conversely, cells lacking Xrn1 exhibited changes in the localization and/or abundance of numerous factors linked to mRNA turnover. Based on these data, we uncovered a new role for cytoplasmic poly(A) binding protein in repressing RNAPII transcription upon its mRNA decay-induced translocation to the nucleus.
HostingRepositoryPRIDE
AnnounceDate2018-11-04
AnnouncementXMLSubmission_2018-11-04_13:53:17.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJoel Federspiel
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-04-13 00:16:21ID requested
12018-11-04 13:53:18announced
Publication List
Gilbertson S, Federspiel JD, Hartenian E, Cristea IM, Glaunsinger B, Changes in mRNA abundance drive shuttling of RNA binding proteins, linking cytoplasmic RNA degradation to transcription. Elife, 7():(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: TMT, translocation, RNA binding proteins, quantitative MS
Contact List
Ileana M Cristea
contact affiliationDepartment of Molecular Biology, Princeton University, USA
contact emailicristea@princeton.edu
lab head
Joel Federspiel
contact affiliationPrinceton University
contact emailjoel.federspiel@princeton.edu
dataset submitter
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Dataset FTP location
PRIDE project URI
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