PXD009484 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Seedling proteome of Arabidopsis thaliana pux10-3 mutants |
Description | Lipid droplets (LD) of seedlings lacking PUX10 stay significantly smaller during germination than the LDs of wild type seedlings. The observation that the removal of a LD-associated protein from a plant affects LD morphology has been observed previously {Siloto et al., 2006}{Gidda et al., 2016}. Especially the effect of the knock-out of the major structural LD protein Oleosin1 leading to a strongly increased size of LDs described by Siloto et al. might indicate that the abundance of structural LD proteins influences the LD size. Thus, the influence of the absence of PUX10 on the abundance of these proteins was investigated via a proteomic approach. Here, proteins isolated from homogenized seedlings of different lines (Ws-4 and pux10-3) and different developmental stages (wet seeds, and 1 to 3 days after imbibition (dai)) were subjected to tryptic digest and LC-MS/MS analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2018-08-13 |
AnnouncementXML | Submission_2018-08-13_12:32:46.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Till Ischebeck |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-04-12 06:00:31 | ID requested | |
⏵ 1 | 2018-08-13 12:32:47 | announced | |
Publication List
Kretzschmar FK, Mengel LA, M, ü, ller AO, Schmitt K, Blersch KF, Valerius O, Braus GH, Ischebeck T, PUX10 Is a Lipid Droplet-Localized Scaffold Protein That Interacts with CELL DIVISION CYCLE48 and Is Involved in the Degradation of Lipid Droplet Proteins. Plant Cell, 30(9):2137-2160(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: seedling, Arabidopsis thaliana, lipid droplet proteins, PUX10 |
Contact List
Dr. Till Ischebeck |
contact affiliation | Department of Plant Biochemistry Georg-August-Universität Göttingen Justus-von-Liebig Weg 11 37077 Göttingen Germany Tel. +49-551-39-14430 |
contact email | tischeb@gwdg.de |
lab head | |
Till Ischebeck |
contact affiliation | University of Goettingen |
contact email | tischeb@gwdg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009484 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009484
- Label: PRIDE project
- Name: Seedling proteome of Arabidopsis thaliana pux10-3 mutants