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PXD009483

PXD009483 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleInner ear exosomes and their potential use as biomarkers
DescriptionExosomes are nanovesicles involved in intercellular communications. They are released by a variety of cell types; however, their presence in the inner ear has not been described in the literature. The aims of this study were to determine if exosomes are present in the inner ear and, if present, characterize the changes in their protein content in response to ototoxic stress. In this laboratory investigation, inner ear explants of 5-day-old Wistar rats were cultured and treated with either cisplatin or gengentamicin. Exosomes were isolated using ExoQuick, serial centrifugation, and mini-column methods. Confirmation and characterization of exosomes was carried out using transmission electron microscopy (TEM), ZetaView, BCA protein analysis, and proteomics. Vesicles with a typical size distribution for exosomes were observed using TEM and ZetaView. Proteomic analysis detected typical exosome markers and markers for the organ of Corti. There was a statistically significant reduction in the exosome protein level and number of particles per cubic centimeter when the samples were exposed to ototoxic stress. Proteomic analysis also detected clear differences in protein expression when ototoxic medications were introduced. This is the first report describing exosomes derived from the inner ear. Because these exosomes varied in number and protein composition when the inner ear was exposed to ototoxic stress, the exciting possibility exists that they might be used as biomarkers to monitor inner ear function.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:44:20.312.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD009483
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlexander Schmidt
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListOxidation; Acetyl; Carbamidomethyl
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-04-12 05:56:06ID requested
12018-07-04 05:30:00announced
22024-10-22 04:44:22announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD009483;
Wong EHC, Dong YY, Coray M, Cortada M, Levano S, Schmidt A, Brand Y, Bodmer D, Muller L, Inner ear exosomes and their potential use as biomarkers. PLoS One, 13(6):e0198029(2018) [pubmed]
10.1371/journal.pone.0198029;
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: exosome, inner ear,label-free quantification
Contact List
Alexander Schmidt
contact affiliationBiozentrum, University of Basel, Switzerland
contact emailalex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliationProteomics Core Facility
contact emailalex.schmidt@unibas.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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