PXD009481

PXD009481 is an original dataset announced via ProteomeXchange.

Title	Human Cdc14A is phosphorylated by Cdk1 at early mitosis
Description	Cdc14 enzymes compose a family of highly conserved phosphatases that are present in a wide range of organisms, including yeast and humans, and that preferentially reverse the phosphorylation of Cyclin-Dependent Kinase (Cdk) substrates. The budding yeast Cdc14 orthologue has essential functions in the control of late mitosis and cytokinesis. In mammals, however, the two Cdc14 homologues, Cdc14A and Cdc14B, do not play a prominent role in controlling late mitotic events, suggesting that some Cdc14 functions are not conserved across species. Moreover, in yeast, Cdc14 is regulated by changes in its subcellular location and by phosphorylation events. In contrast, little is known about the regulation of human Cdc14 phosphatases. Here, we have studied how the human Cdc14A orthologue is regulated during the cell cycle. We found that Cdc14A is phosphorylated on Ser411, Ser453 and Ser549 by Cdk1 early in mitosis and becomes dephosphorylated during late mitotic stages. Interestingly, in vivo and in vitro experiments revealed that, unlike in yeast, Cdk1-mediated phosphorylation of human Cdc14A did not control its catalytic activity but likely modulated its interaction with other proteins in early mitosis. These findings point to differences in Cdk1-mediated mechanisms of regulation between human and yeast Cdc14 orthologues.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:35.876.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009481
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nieves Ibarrola
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Phospho; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-12 03:54:51	ID requested
1	2018-08-13 11:05:27	announced
⏵ 2	2024-10-22 04:44:38	announced	2024-10-22: Updated project metadata.

10.6019/PXD009481;

10.1038/s41598-018-30253-8;

Ovejero S, Ayala P, Malumbres M, Pimentel-Mui, ñ, os FX, Bueno A, Sacrist, á, n MP, Biochemical analyses reveal amino acid residues critical for cell cycle-dependent phosphorylation of human Cdc14A phosphatase by cyclin-dependent kinase 1. Sci Rep, 8(1):11871(2018) [pubmed]

curator keyword: Biological

submitter keyword: Molecular biology, Cyclin-dependent kinase (CDK), Cdc14.,Cell cycle, Phosphatase, Phosphorylation

María P. Sacristan
contact affiliation	-Instituto de Biología Molecular y Celular del Cáncer, Universidad de Salamanca/CSIC, 37007 Salamanca, Spain. -Departamento de Microbiología y Genética, Universidad de Salamanca, 37007 Salamanca, Spain.
contact email	msacristan@usal.es
lab head
Nieves Ibarrola
contact affiliation	Center for Cancer Research
contact email	nibarrola@usal.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009481

PRIDE project URI

• PRIDE
  1. PXD009481
     1. Label: PRIDE project
     2. Name: Human Cdc14A is phosphorylated by Cdk1 at early mitosis