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PXD009481

PXD009481 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman Cdc14A is phosphorylated by Cdk1 at early mitosis
DescriptionCdc14 enzymes compose a family of highly conserved phosphatases that are present in a wide range of organisms, including yeast and humans, and that preferentially reverse the phosphorylation of Cyclin-Dependent Kinase (Cdk) substrates. The budding yeast Cdc14 orthologue has essential functions in the control of late mitosis and cytokinesis. In mammals, however, the two Cdc14 homologues, Cdc14A and Cdc14B, do not play a prominent role in controlling late mitotic events, suggesting that some Cdc14 functions are not conserved across species. Moreover, in yeast, Cdc14 is regulated by changes in its subcellular location and by phosphorylation events. In contrast, little is known about the regulation of human Cdc14 phosphatases. Here, we have studied how the human Cdc14A orthologue is regulated during the cell cycle. We found that Cdc14A is phosphorylated on Ser411, Ser453 and Ser549 by Cdk1 early in mitosis and becomes dephosphorylated during late mitotic stages. Interestingly, in vivo and in vitro experiments revealed that, unlike in yeast, Cdk1-mediated phosphorylation of human Cdc14A did not control its catalytic activity but likely modulated its interaction with other proteins in early mitosis. These findings point to differences in Cdk1-mediated mechanisms of regulation between human and yeast Cdc14 orthologues.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:44:35.876.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD009481
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterNieves Ibarrola
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListPhospho; Oxidation; Carbamidomethyl
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-04-12 03:54:51ID requested
12018-08-13 11:05:27announced
22024-10-22 04:44:38announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD009481;
10.1038/s41598-018-30253-8;
Ovejero S, Ayala P, Malumbres M, Pimentel-Mui, ñ, os FX, Bueno A, Sacrist, á, n MP, Biochemical analyses reveal amino acid residues critical for cell cycle-dependent phosphorylation of human Cdc14A phosphatase by cyclin-dependent kinase 1. Sci Rep, 8(1):11871(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Molecular biology, Cyclin-dependent kinase (CDK), Cdc14.,Cell cycle, Phosphatase, Phosphorylation
Contact List
María P. Sacristan
contact affiliation-Instituto de Biología Molecular y Celular del Cáncer, Universidad de Salamanca/CSIC, 37007 Salamanca, Spain. -Departamento de Microbiología y Genética, Universidad de Salamanca, 37007 Salamanca, Spain.
contact emailmsacristan@usal.es
lab head
Nieves Ibarrola
contact affiliationCenter for Cancer Research
contact emailnibarrola@usal.es
dataset submitter
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Dataset FTP location
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PRIDE project URI
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