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PXD009476

PXD009476 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSite-specific Extraction of O-linked glycopeptides (EXoO) Maps Landscape of In Vivo O-linked Glycoproteome
DescriptionProtein glycosylation is one of the most common protein modifications and plays essential roles in biology and therapeutics. However, the analysis of in vivo O-linked glycosylation, a major type of protein glycosylation, has been severely impeded by the scarcity of technology. Here, a chemoenzymatic method was presented for the site-specific extraction of O-linked glycopeptides (EXoO), which achieved simultaneous enrichment and unambiguous mapping of over 3,000 O-linked glycosylation sites and corresponding O-linked glycans on over 1,000 proteins in human kidney tissues, serum and T cells. The large-scale localization of O-linked glycosylation sites nearly doubles the sites identified in the last decades demonstrating that EXoO is the most effective method to-date for defining the site-specific O-linked glycoproteome in different types of sample. Structural analysis of the sites revealed conserved motifs and topological orientation facing extracellular space or lumen of ER and Golgi. Striking signature of aberrant in vivo O-linked glycoproteome was observed between kidney tumor and normal tissues discovering key factors in tumor biology. The O-linked glycoproteome play diverse roles on the ER, Golgi membrane, cell surface and extracellular space arguing that EXoO can be applied broadly to the analysis of O-linked glycoproteins in biology and therapeutics.
HostingRepositoryPRIDE
AnnounceDate2020-07-22
AnnouncementXMLSubmission_2020-07-21_18:20:49.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWeiming Yang
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListcomplex glycosylation
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-04-12 02:05:03ID requested
12018-12-05 03:05:34announced
22020-07-21 18:20:51announced2020-07-22: Updated publication reference for PubMed record(s): 30459171, 32681153.
Publication List
Yang W, Song A, Ao M, Xu Y, Zhang H, Large-scale site-specific mapping of the O-GalNAc glycoproteome. Nat Protoc, 15(8):2589-2610(2020) [pubmed]
Yang W, Ao M, Hu Y, Li QK, Zhang H, Mapping the O-glycoproteome using site-specific extraction of O-linked glycopeptides (EXoO). Mol Syst Biol, 14(11):e8486(2018) [pubmed]
Keyword List
curator keyword: Technical
submitter keyword: Site-specific, in vivo, O-linked, glycosylation, EXoO
Contact List
Hui Zhang
contact affiliationDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
contact emailhuizhang@jhu.edu
lab head
Weiming Yang
contact affiliationJohns Hopkins University
contact emailwesley.young@hotmail.com
dataset submitter
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