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PXD009460

PXD009460 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterization of disulfide linkages in proteins by 193 nm ultraviolet photodissociation (UVPD)
DescriptionInter-linked disulfide bonds connecting peptide chains are homolytically cleaved with 193 nm ultraviolet photodissociation (UVPD). Analysis of insulin demonstrates the ability for UVPD to cleave multiple disulfide bonds and provide sequence coverage of multiple peptide chains in the same MS/MS event. The information provided by UVPD of disulfide-bound peptides is comparable to information garnered from other high energy dissociation methods but requires an activation period an order of magnitude faster than these methods. This phenomenon was investigated, along with a partial reduction and alkylation sample preparation to determine disulfide connectivities of enzymatically digested proteins. The 4 disulfide bonds of lysozyme and the 19 disulfide bonds of serotransferrin were unambiguously characterized through non-reduced and partially reduced protein digestions. 
HostingRepositoryjPOST
AnnounceDate2018-04-28
AnnouncementXMLSubmission_2019-10-09_17:03:21.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterM. Montana Quick
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Gallus gallus; NCBI TaxID: 9031; scientific name: Bos taurus; NCBI TaxID: 9913;
ModificationListunknown modification; N-ethylmaleimide derivatized cysteine
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-04-10 13:18:06ID requested
12018-04-27 10:25:08announced
22018-09-19 00:54:35announcedUpdated CV, FTP location and Modification list.
32019-10-09 17:03:25announced2019-10-10: Updated PubMed.
Publication List
Quick MM, Crittenden CM, Rosenberg JA, Brodbelt JS, Characterization of Disulfide Linkages in Proteins by 193 nm Ultraviolet Photodissociation (UVPD) Mass Spectrometry. Anal Chem, 90(14):8523-8530(2018) [pubmed]
Keyword List
submitter keyword: Disulfide bond, Mass spectrometry, ultraviolet photodissociation, UVPD, HPLC
Contact List
Jennifer S. Brodbelt
lab head
M. Montana Quick
contact affiliationUniversity of Texas at Austin
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location