<<< Full experiment listing

PXD009451

PXD009451 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomics identification of seminal fluid proteins in male Drosophila melanogaster
DescriptionSeminal fluid contains some of the fastest evolving proteins currently known. These seminal fluid proteins (Sfps) play crucial roles in reproduction, such as supporting sperm function, and – particularly in insects – modifying female physiology and behaviour. Identification of Sfps in small animals is challenging, and often relies on samples taken from the female reproductive tract after mating. A key pitfall of this method is that it might miss Sfps that are of low abundance due to dilution in the female-derived sample or rapid processing in females. Here we present a new and complimentary method, which provides added sensitivity to Sfp identification. We applied label-free quantitative proteomics to Drosophila melanogaster male reproductive tissue – where Sfps are unprocessed, and highly abundant – and quantified Sfps before and immediately after mating, to infer those transferred during copulation. We also analysed female reproductive tracts immediately before and after copulation to confirm the presence and abundance of known and candidate Sfps, where possible. Results were cross-referenced with transcriptomic and sequence databases to improve confidence in Sfp detection. Our data was consistent with 124 previously reported Sfps. We found 8 high-confidence novel candidate Sfps, which were both depleted in mated versus unmated males and identified within the reproductive tract of mated but not virgin females. We also identified 31 more candidates that are likely Sfps based on their abundance, known expression and predicted characteristics, and revealed that four proteins previously identified as Sfps are at best minor contributors to the ejaculate. The estimated copy numbers for our candidate Sfps were lower than for previously identified Sfps, supporting the idea that our technique provides a deeper analysis of the Sfp proteome than previous studies. Our results demonstrate a novel, high-sensitivity approach to the analysis of seminal fluid proteomes, whose application will further our understanding of reproductive biology.
HostingRepositoryPRIDE
AnnounceDate2018-12-19
AnnouncementXMLSubmission_2018-12-19_08:32:56.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPhilip Charles
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListS-carboxamidoethyl-L-cysteine; monohydroxylated residue; deamidated residue
InstrumentQ Exactive HF; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-04-10 03:01:00ID requested
12018-12-19 08:32:57announced
Publication List
Sepil I, Hopkins BR, Dean R, Th, é, z, é, nas ML, Charles PD, Konietzny R, Fischer R, Kessler BM, Wigby S, . Mol Cell Proteomics, 18(Suppl 1):S46-S58(2019) [pubmed]
Keyword List
submitter keyword: Drosophila melanogaster
Seminal fluid
LFQ
Contact List
Stuart Wigby
contact affiliationEdward Grey Institute, Department of Zoology, University of Oxford, Oxford, UK
contact emailstuart.wigby@zoo.ox.ac.uk
lab head
Philip Charles
contact affiliationUniversity of Oxford
contact emailphilip.charles@ndm.ox.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/12/PXD009451
PRIDE project URI
Repository Record List
[ + ]