PXD009450

PXD009450 is an original dataset announced via ProteomeXchange.

Title	Functional Proteomic Profiling of Secreted Serine Proteases in Health and Inflammatory Bowel Disease
Description	Dysregulated proteolysis plays a pivotal role in the pathophysiology of inflammatory bowel disease. Nonetheless, the identity of overactive proteases released by human colonic mucosa remains unknown. Herein, we employed a serine protease-targeted activity-based probe (ABP) coupled with mass spectral analysis to identify active forms of proteases secreted by the colonic mucosa of healthy volunteers and inflammatory bowel disease patients. With this approach, we identified seven active serine proteases: cathepsin G, plasma kallikrein, plasmin, tryptase, chymotrypsin-like elastase 3A, aminopeptidase B, and thrombin. Furthermore, cathepsin G and thrombin were overactive in supernatants from inflammatory bowel disease patients once compared to healthy volunteers.
HostingRepository	PRIDE
AnnounceDate	2018-05-18
AnnouncementXML	Submission_2018-05-22_05:53:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emmanuelle Mouton Barbosa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-10 01:54:51	ID requested
1	2018-05-18 06:58:58	announced
⏵ 2	2018-05-22 05:53:30	announced	Updated publication reference for PubMed record(s): 29777136.

Denadai-Souza A, Bonnart C, Tapias NS, Marcellin M, Gilmore B, Alric L, Bonnet D, Burlet-Schiltz O, Hollenberg MD, Vergnolle N, Deraison C, Functional Proteomic Profiling of Secreted Serine Proteases in Health and Inflammatory Bowel Disease. Sci Rep, 8(1):7834(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: serine protease, inflammatory bowel disease, inflammation, functional proteomics, activity-based probe

Alexandre Denadai-Souza
contact affiliation	INSERM IRSD U1220 CHU PURPAN PLACE DU DOCTEUR BAYLAC CS 60039 31024 TOULOUSE CEDEX 3 ​FRANCE
contact email	alexandre.denadai-souza@kuleuven.be
lab head
Emmanuelle Mouton Barbosa
contact affiliation	IPBS CNRS
contact email	emmanuelle.mouton@ipbs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD009450

PRIDE project URI

• PRIDE
  1. PXD009450
     1. Label: PRIDE project
     2. Name: Functional Proteomic Profiling of Secreted Serine Proteases in Health and Inflammatory Bowel Disease