PXD009444

PXD009444 is an original dataset announced via ProteomeXchange.

Title	A chemical probe to monitor parkinsonism-associated protein DJ-1 in live cells
Description	Oxidative stress is a contingent trigger of Parkinson`s disease (PD). DJ-1, a protein involved in oxidative stress sensing, gained major attention when mutations in its gene were identified in PD patients. Although the study of the corresponding protein function is still in its infancy, a crucial oxidation sensor at a conserved cysteine was linked to the disease. Inspired by inhibition studies with a bacterial homolog of DJ-1 we here screen several amino-epoxycylcohexenones and identify a chemical probe that exhibits specificity for the human protein in various cell lines. The probe selectively labeled the oxidation sensor via nucleophilic attack of the conserved cysteine onto the epoxide ring. This covalent addition occurred only in the reduced state. Whole proteome studies in HeLa, A549 and SHSY5Y cell lines confirmed strong enrichment of solely reduced DJ-1 which was diminished by increasing oxidative stress. The probe thus facilitates the first selective in situ monitoring of DJ-1 in its reduced state and enables studying the function of this important biomarker in dependence of oxidative stress.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:30.479.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonas Drechsel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL; Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-09 08:37:48	ID requested
1	2018-07-17 01:43:02	announced
2	2018-07-30 01:28:11	announced	Updated publication reference for PubMed record(s): 30011180.
⏵ 3	2024-10-22 04:46:48	announced	2024-10-22: Updated project metadata.

Drechsel J, Mandl FA, Sieber SA, Chemical Probe To Monitor the Parkinsonism-Associated Protein DJ-1 in Live Cells. ACS Chem Biol, 13(8):2016-2019(2018) [pubmed]

10.1021/acschembio.8b00633;

curator keyword: Biomedical

submitter keyword: Human, LC-MS/MS

Stephan A Sieber
contact affiliation	Center for Integrated Protein Science Munich (CIPSM) at the Department of Chemistry, Technische Universität München, Lichtenbergstraße 4, 85747 Garching b. München ( lab head )
contact email	stephan.sieber@tum.de
lab head
Jonas Drechsel
contact affiliation	TU Munich
contact email	jonas.drechsel@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD009444

PRIDE project URI

• PRIDE
  1. PXD009444
     1. Label: PRIDE project
     2. Name: A chemical probe to monitor parkinsonism-associated protein DJ-1 in live cells